1H-Imidazolium, 1,3-diethyl-, acetate (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,3-diethyl-1H-imidazol-3-ium acetate

EC Number:

692-759-5

Cas Number:

1040916-84-4

Molecular formula:

C7 H13 N2 . C2 H3 O2

IUPAC Name:

1,3-diethyl-1H-imidazol-3-ium acetate

Details on test material:

- Name of test material (as cited in study report): EEIM Acetate
- Physical state: liquid
- Purity: 97.4%
- Lot/batch No.: 4-5441-2379 VTA
- Expiration date of the lot/batch: Dec 2012

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since EEIM Acetate is a water soluble substance, the test concentrations were prepared by dilution of a 100 mg/l stock solution of the test material in aerated medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Test organism: Daphnia magna (Straus, 1820)
- Breeding:Parental and young Daphnia held in 4 l glass aquaria (3 l medium) at 20 ± 2 °C
- Illumination: 16 h per day
- Medium: Continuously aerated Elendt M4 medium prepared with deionised water
- Feed: Suspension of Desmodesmus subspicatus in Elendt M4 medium with an optical density OD680 of about 15 units
- Frequency of feeding while breeding 400 μl per Daphnia per day, except on weekends
- Control of sensitivity Acute reference test with potassium dichromate conducted twice a year

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 2 °C

pH:

7.1-7.6

Dissolved oxygen:

5.2-6.8 mg/L

Details on test conditions:

Nominal concentrations (verified analytically): 3.13, 6.25, 12.5, 25, 50 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

25.8 mg/L

Nominal / measured:

nominal

Conc. based on:

other: EEIM cation

Basis for effect:

mobility

Remarks on result:

other: Nominal concentratiolns verified analytically.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

43.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Nominal concentrations verified analytically

Details on results:

- Behavioural abnormalities: none
- Other biological observations: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Acutely harmful to aquatic invertebrates. As the acetate anion is of very low toxicity effects are assuemd to be caused by the EEIM cation.