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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study without restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-diethyl-1H-imidazol-3-ium acetate
EC Number:
692-759-5
Cas Number:
1040916-84-4
Molecular formula:
C7 H13 N2 . C2 H3 O2
IUPAC Name:
1,3-diethyl-1H-imidazol-3-ium acetate
Details on test material:
- Name of test material (as cited in study report): EEIM Acetate
- Physical state: liquid
- Purity: 97.4%
- Lot/batch No.: 4-5441-2379 VTA
- Expiration date of the lot/batch: Dec 2012

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since EEIM Acetate is a water soluble substance, the test concentrations were prepared by dilution of a 100 mg/l stock solution of the test material in aerated medium
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Collection of Algal Cultures, Institute of Freshwater Ecology, University of Göttingen, D-37073 Göttingen, Germany
- Method of cultivation: Axenic slope culture

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20.9-22.9
pH:
7.7-8.3
Nominal and measured concentrations:
12.5, 25, 50, 100 and 200 mg/L nominal, verified analytically.
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 ml flasks, all-glass, with 100 ml of test medium, shaken (about 130 rpm)
- Material, size, headspace, fill volume: 100 mL
- Aeration: none
- Initial cells density: 0.50–0.85 µg/ml with respect to dry weight corresponding to about 2–5x103 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
41.1 mg/L
Nominal / measured:
nominal
Conc. based on:
other: EEIM cation
Basis for effect:
growth rate
Remarks on result:
other: Nominal concenrations verified analytically
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
65.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Nominal concentrations verified analytically
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
66.3 mg/L
Nominal / measured:
nominal
Conc. based on:
other: EEIM cation
Basis for effect:
growth rate
Remarks on result:
other: Nominal concenrations verified analytically
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
105 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Nominal concenrations verified analytically
Details on results:
- Exponential growth in the control (for algal test): yes
- Effect concentrations exceeding solubility of substance in test medium: no

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EEIM acetate is with high probability not harmful to algae. The intrinsic toxicity of the substance arise from the EEIM cation as the acetate anion is expected to be of low toxicity.