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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

EC50(3h) > 1000 mg/l (nominal)

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

The influence of the test item on the respirationrate of activated sludge was investigated after a contact time of 3 hours according to the OECD 209 and EU C.11

The preliminary test was performed using 3 concentrations 10, 100 and 1000 mg/l. Further two blank controls, the three abiotic controls with the highest concentration of the test item was included and two beakers with specific nitrification inhibitor to determine the nitrification potential of sludge. 3,5-dichlorophenol was used as reference item. Three concentrations in geometric progression with a factor of 2.0 (5, 10 and 20 mg/l) were selected. The abiotic decomposition was not detected even at the highest concentration of the test item, no correction of results for abiotic oxygen uptake was needed. The nitrifying activity of the activated sludge was detected in the preliminary test. 

In this preliminary test, the highest total inhibitory effect of the test item is 0 %, so the activity of nitrifying have not to be taken into account. The EC50 for inhibition the respiration rate could not be calculated. 

The absence of inhibition of oxygen consumption by the test item in the preliminary test demonstrated that a definitive test is unnecessary.

The result could be used as final one, because preliminary test design includes three replicates required by guidelines. Exact value of EC50 could not be calculated, the value of EC50is given in the form of a range:

EC50(3h) > 1000 mg/l