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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:
freshwater

Additional information

The aerobic biodegradability of the test item was evaluated in the CO2Evolution Test was performed following OECD 301B resp. EU C.4-C.

Two experiments were performed. In the first experiment one of the validity criteria wasn’t met. The difference between the test replicates was 29.8 % instead of < 20 % as stated in the guidelines. Therefore, the study was repeated, under the same test conditions.

The test item was tested using a concentration of nominally 20 mg organic carbon/l in test medium for 28 days. Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/l).

Toxicity, abiotic, positive and blank controls run in parallel. Analyses of the emitted CO2 were made by IC measurement. All validity criteria were met. Degradation of the positive control surpassed the pass level of 60 % after 6 days.

The following data were determined for the test item:

10-day-window: day 9 – 19; degradation at the end of 10-day-window 14 %; degradation at the end of the test 17 %.

The test item is not readily biodegradable following OECD 301B and EU C.4-C respectively. As degradation did not reach the pass level of 60% in the course of the test, the substance is considered as not ultimately biodegradable, within 28 days as well.