Reaction mass of lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2,7-disulfonate and lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-{[4-(vinylsulfonyl)phenyl]diazenyl}naphthalene-2,7-disulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 08, 1986 to September 07, 1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

The study was conducted according to an internal standard method, with quality assurance but not under GLP. No analytical determination of test concentrations.

Principles of method if other than guideline:

6-24 d old Daphnia magna were exposed to the test substance in a static system for a duration of 24 h. The exposure doses were equivalent to 0, 300, 400 and 500 mg/L (nominal). The positive control potassium dichromate was tested in parallel at 0.75, 1.0, 1.25, 1.50, 1.75, 2.0, 3.0 and 5.0 mg/L. There were 5 Daphnia per 10 mL vessel and 4 vessels per test concentration.

The number of immobile Daphnia was recorded at 24 h. Oxygen concentration, pH and temperature were measured in each of the test vessels.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Test temperature:

19.5 - 21°C

pH:

7.9-8.2

Dissolved oxygen:

8.5-8.7 mg/L

Nominal and measured concentrations:

- Nominal concentration: 0, 300, 400 and 500 mg/L

Details on test conditions:

- Test vessel: Cylindrical test vessels (diameter 2.6 cm, height 6.2 cm)
- Volume: 10 mL test medium

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

>= 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The 24 h EC0, EC50 and EC 100 for the reference substance were 0.75, 1.92 and 5.0 mg/L, respectively.

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the nominal 24 h EC50 of the test substance was determined to be >500 mg/L.

Executive summary:

A test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to an internal standard operating procedure. In this test, 4 replicates of 5 daphnids were exposed to the substance at nominal concentrations of 0, 300, 400 and 500 mg/L under static conditions for 24 h. Immobility was recorded at 24 h, along with measurement of oxygen concentration, pH and temperature. The positive control potassium dichromate was tested in parallel at 0.75, 1.0, 1.25, 1.50, 1.75, 2.0, 3.0 and 5.0 mg/L. 1 daphnid each died at 400 and 500 mg/L. Therefore, under the study conditions, the nominal 24 h EC50 of the test substance was determined to be >500 mg/L (Caspers, 1986).

Description of key information

The nominal 24 h EC50 of the test substance was determined to be >500 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

500 mg/L

Additional information

A test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to an internal standard operating procedure. In this test, 4 replicates of 5 daphnids were exposed to the test substance at nominal concentrations of 0, 300, 400 and 500 mg/L under static conditions for 24 h. Immobility was recorded at 24 h, along with measurement of oxygen concentration, pH and temperature. The positive control potassium dichromate was tested in parallel at 0.75, 1.0, 1.25, 1.50, 1.75, 2.0, 3.0 and 5.0 mg/L. 1 daphnid each died at 400 and 500 mg/L. Therefore, under the study conditions, the nominal 24 h EC50 of the test substance was determined to be >500 mg/L (Caspers, 1986).