Reaction mass of lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2,7-disulfonate and lithium sodium hydrogen 4-amino-6-({5-[(5-chloro-2,6-difluoropyrimidin-4-yl)amino]-2-sulfonatophenyl}diazenyl)-5-hydroxy-3-{[4-(vinylsulfonyl)phenyl]diazenyl}naphthalene-2,7-disulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

A study was conducted to determine the toxicity of the test substance to Zebra fish (Danio rerio). The fish were exposed for 96 h to nominal concentrations of 0, 300, 400 and 500 mg/L. Mortality and symptoms were assessed daily.

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: West Aquarium
- Length at study initiation (range): 2.5-3.5 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

Exposed test animals were observed for behavioural changes and mortality at 0, 2, 24, 48, 72 and 96 h

Test temperature:

22.8°C

pH:

6.6

Dissolved oxygen:

8.8 mg/L

Nominal and measured concentrations:

Nominal: 0, 300, 400 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Type (delete if not applicable): open
- Material, fill volume: glass, 5 L
- Aeration: yes
- No. of organisms per vessel: ten
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.3

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the 96 h LC0 of the test substance to Zebra fish (Brachydanio rerio) was determined to be >500 mg/L (nominal).

Executive summary:

A study was conducted to determine the toxicity of the test substance to Zebra fish (Danio rerio) according to an internal protocol. The fish were exposed for 96 h to nominal test substance concentrations of 0, 300, 400 and 500 mg/L. Mortality and symptoms were assessed daily. The analytical verification was conducted by photometry (colour strength at 300, 400 and 500 mg/L). No treatment-related adverse symptoms were observed in any group. No mortality was observed among the exposed test animals of any group. Under the study conditions, the 96 h LC0 and LC50 of the test substance to Zebra fish (Danio rerio) were determined to be >500 mg/L (nominal) (Kolsche, 1986).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L

Additional information

A study was conducted to determine the toxicity of the test substance to Zebra fish (Danio rerio) according to an internal protocol. The fish were exposed for 96 h to nominal test substance concentrations of 0, 300, 400 and 500 mg/L. Mortality and symptoms were assessed daily.The analytical verification was conducted by photometry (colour strength at 300, 400 and 500 mg/L). No treatment-related adverse symptoms were observed in any group. No mortality was observed among the exposed test animals of any group. Under the study conditions, the 96 h LC0 and LC50 of the test substance to Zebra fish (Brachydanio rerio) were determined to be >500 mg/L (nominal) (Kolsche, 1986).