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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to an internal SOP and with quality assurance review. In this test, 4 replicatesof 5 daphnids were exposed to the substance at a nominal concentration of 0, 300, 400 and 500 mg/L under static conditions for 24 h.Immobility was recorded at 24 h, alog with oxygen concentration, pH and temperature. The positive control potassium dichromate was tested in parallel at 0.75, 1.0, 1.25, 1.50, 1.75, 2.0, 3.0 and 5.0 mg/L. Under the test conditions, the 24 h EC0 of the test substance was >=500 mg/L (nominal) (Caspers, 1986).

A study was conducted to determine the acute toxicity of the test substance to the algae Desmodesmus subspicatus according to EU Method C.3, and an instruction for the performance of a modified algal growth inhibition test published by Memmert & Knoell (RCC Umweltchemie, August 1992), in compliance with GLP. Triplicate inocula of the algae were exposed to nominal concentrations of the test substance (0.4, 1.0, 2.3, 4.7, 10.3, 22.7 and 50 mg/L) for 72 h under static conditions. Quantification of the applied concentrations was carried out using HPLC. A second series (Part II) of test vessels treated in the same way as the controls (Part III), but placed below glass vessels containing the same range of concentrations of the test substance as described above, but without algae, was set up to distinguish between algistatic (indirect effect caused by light absorption) and algicidal (toxic) effects. The cell densities were measured at 24, 48 and 72 h. Inhibition of the algal population was measured as reduction in growth and growth rate, relative to control cultures grown under identical conditions. The measured concentrations of the test substance ranged from 102-117% of nominal values at 0 h, and from 73-113% of nominal values at 72 h. Under the conditions of the study, the 72 h EbC10 (biomass) and ErC10 (growth rate) for Desmodesmus subspicatus were determined to be 0.4 and 0.7 mg/L (nominal concentrations) respectively; the 72 h EbC50 and ErC50 were determined to be 2.9 and 6.8 mg/L (nominal concentrations) respectively; and the NOECs for biomass and growth rate were determined to be <0.4 and 0.4 mg/L (nominal concentration) respectively. A comparison of the results for the parameter "percentage inhibition of the growth rate" gained in Parts I and II of the study, results in differences exceeding 10% only at the highest test substance concentration (50 mg/L). At this concentration, the difference observed for this parameter was 14.7%. Due to the decreasing accuracy of the cell number determination procedure at very low levels, this difference was not considered as a difference between Parts I and II of the study. Therefore the inhibition of growth observed in this study was considered to be caused by light absorption only (Bruns, 2001).