2,5-bis(1,1,3,3-tetramethylbutyl)hydroquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Range finding test for Study acc. to OECD 305

Principles of method if other than guideline:

Deviation from test plan: the amount of feed in the test plan was 2% of the fish weight, but it was actually 1.5%.

GLP compliance:

not specified

Remarks:

Range finding test for GLP Study acc. to OECD 305

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Japanese rice fish
- Source: bred at testing facility
- Length at study initiation (length definition, mean, range and SD): 2 ± 1 cm
- Weight at study initiation (mean and range, SD): ca. 0.2 g


ACCLIMATION
- Acclimation period: 6 wk
- Acclimation conditions (same as test or not): flow-through conditions
- Type and amount of food during acclimation: Tertramin fishfood (Tetra)
- Feeding frequency during acclimation: dayil, approx. 1.5 % of the body weight (no feeding 24 hours before the test)
- Health during acclimation (any mortality observed): <5% dead fish during 1 week before test (no medication of fish)

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

24± 1 °C

Nominal and measured concentrations:

5 mg/L

Details on test conditions:

TEST SYSTEM
details on test system are not available


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Yokohama city tap water was dechlorinated by activated carbon filtration and sodium thiosulfate addition and used as test water.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Description of key information

Not acutely toxic to fish

Additional information

The assessment of the acute toxicity to fish is based on the results of a range finding study performed in the framework of a bioaccumulation study following OECD 305. Fish of the species Oryzias latipes were exposed to a substance concentration of 5 mg/L in a 96 hour flow-through test. Mortalities were not observed. The determined LC50 (96 h) was >5 mg/L.