1-Propanaminium, 3,3',3''-[phosphinylidynetris(oxy)]tris[N-(3-aminopropyl)-2-hydroxy-N,N-dimethyl-, N,N',N''-tri-C6-18 acyl derivs. trichlorides

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

3 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

OECD 209 guideline followed to GLP, June 2016
Assessed to be scientifically valid

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Specific details on test material used for the study:

COCAMIDOPROPYL PG - DIMONIUM CHLORIDE PHOSPHATE; LOT # 50113A16
Date and Quantity Received: 07 Mar 16
Physical Description: Clear liquid
Storage Conditions: Room temperature
Purity: 41% actives in water

Analytical monitoring:

not required

Vehicle:

no

Details on test solutions:

Substance diluted directly into water.

Test organisms (species):

activated sludge

Details on inoculum:

Samples of activated sludge were obtained from the Brazos Valley Sewer (Sugar Land, Texas) and aerated during transport and storage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

pH:

Buffered to pH 7.0

Nominal and measured concentrations:

Nominal 62.5, 125, 250, 500, 1000 mg/l actives

Reference substance (positive control):

yes

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

EC100

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

EC0

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth inhibition

Details on results:

At concentrations up to and including 500 mg/l, there was an enhancement of oxygen consumption
The test report cites a calculated EC50 of 495 mg/l that is clearly incorrect in view of enhanced effects seen at 500 mg/l.

Results with reference substance (positive control):

Considered vlaid.

Validity criteria fulfilled:

yes

Conclusions:

The substance enhanced microbial activity at up to 500 mg/l actives in the main test (although in a range finder, there was up to 25% inhibition noted at 100 mg/l). At 1000 mg/l in the range finder and main studies, there was a clear inhibition, with over 90% inhibition in some replicates. The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l

Executive summary:

The results suggest that the substance should be readily biodegrable at concentrations below 500 mg/l actives in view of enhanced activity; however, at higher concentrations, there appears to be significant inhibition.

The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l

Description of key information

OECD 209 Sludge inhibition

The results suggest that the substance should be readily biodegrable at concentrations below 500 mg/l actives in view of enhanced activity; however, at higher concentrations, there appears to be significant inhibition.

The EC50 is not possible to calculate, but is in the range 500 - 1000 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:

500 mg/L

EC10 or NOEC for microorganisms:

500 mg/L