1-Propanaminium, 3,3',3''-[phosphinylidynetris(oxy)]tris[N-(3-aminopropyl)-2-hydroxy-N,N-dimethyl-, N,N',N''-tri-C6-18 acyl derivs. trichlorides

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

48 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Performed to OECD guidelines to GLP in 2016.
Analysis performed to verify dose levels

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

COCAMIDOPROPYL PG - DIMONIUM CHLORIDE PHOSPHATELOT # 50113A16
Date 07 Mar 16;Physical Description: Clear liquid
Storage Conditions: Room temperature
Purity: 41% active ingredient
Stability: Not provided to testing facility

Analytical monitoring:

yes

Details on sampling:

Dose verification was performed during the study on test solutions sampled at 0 and 48 hours for therange-finding and definitive tests

Vehicle:

no

Details on test solutions:

Readily soluble in water

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magnaSource: STILLMEADOW, Inc. culture laboratory
Age: No greater than 24 hours old at dosing;

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Total hardness 200 mg/L CaCO3

Test temperature:

20 C

pH:

pH 7.9

Dissolved oxygen:

60 - 100% saturation

Nominal and measured concentrations:

Range-finding concentrations of 0.01, 0.1, 1, 10 and 100 mg/l a.i.
Definitive 0, 0.625, 1.25, 2.5, 5.0, 10.0 mg/l ai

Details on test conditions:

250 mL glass beakers; filled with 200 ml of the appropriate solution

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 3 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 0.625 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

Nominal concentrations are reported, based on active component

Validity criteria fulfilled:

yes

Conclusions:

Crude analysis was performed to verify dose levels; since the substance is functionally stable in water and highly soluble, this minimal analysis is not considered sufficient to confirm exposure. Nominal concentrations are cited. A good dose-response relationship was seen with the highest concentrations resulting in 100% immobilisaiton and the lowest having no effect. The EC50 is clearly in the range 1 - 10 mg/l for purposes of classification

Executive summary:

A good dose-response relationship was seen with the highest concentrations resulting in 100% immobilisaiton and the lowest having no effect. The EC50 is clearly in the range 1 - 10 mg/l for purposes of classification

Description of key information

A good dose-response relationship was seen with the highest concentrations resulting in 100% immobilisaiton and the lowest having no effect. The EC50 is clearly in the range 1 - 10 mg/l for purposes of classification

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3 mg/L