2,3-epoxybutane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test procedure in accordance with German national standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38 412, L15 (1982)

Principles of method if other than guideline:

Statistical methods: Probit analysis

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2-epoxybutane

EC Number:

203-438-2

EC Name:

1,2-epoxybutane

Cas Number:

106-88-7

Molecular formula:

C4H8O

IUPAC Name:

2-ethyloxirane

Details on test material:

CHEMICAL NAME: 1,2-epoxybutane
PURITY: >99%

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- species: Golden Orfe (Leuciscus idus L., golden variety)
- supplier: Fischzucht Paul Eggers, D-2354 Hohenwestedt, GER
- age: no data
- length: 7.7 cm (range: 6.9 - 8.9)
- weight: 4.3 g (range: 2.8 - 6.6)
- loading: 4.3 (g fish / L test water)
- pretreatment: twice with 0.05 mg/L malachite green chloride, once with 10 mg/L tetracycline hydrochloride

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 °C

pH:

7.6 - 8.0

Dissolved oxygen:

6.0 - 8.6 mg/L

Details on test conditions:

TEST PROCEDURE
- static
- the product was added to the test water without any pretreatment, subsequently the fish were placed into the aquaria
- nominal concentrations: 46.4, 100, 215, 464 and 1000 mg/L
- exposure vessel type: all-glass aquaria (30 cm x 22 cm x 24 cm), slight aeration
- photoperiod: 16 hours light and 8 hours darkness
- one vessel per concentration/control, 10 fish per replicate
- Water chemistry in test

TEST WATER
- reconstituted freshwater according to DIN 38 412; part 11, October 1982
- preparation from fully demineralised tap water
- conductivity: max. 10 µmho/cm
- The water is carried out by adding
294.0 mg/L CaCl2 x 2 H20
123.3 mg/L MgS04 x 7 H20
63.0 mg/L NaHCO3
5.5 mg/L KCl
- continuous aeration with oil free air
- total hardness: 2.5 mmol/L
- acid capacity: 0.8 mmol/L
- ratio Ca/Mg ions: 4:1
- ratio Na/K ions: 10:1
- pH: about 8.0

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

< 215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

- biological observations: at the concentration of 1000 mg/L after 4 hours tumbling of the fish were observed

- cumulative mortality:
(mg/L) 1h 24h 48h 72h 96h
0 0 0 0 0 0
46.4 0 0 0 0 0
100 0 1 1 1 1
215 0 10 10 10 10
464 0 10     10 10 10
1000 0 10     10 10 10

- lowest test substance concentration causing 100% mortality: 215 mg/L
- mortality of controls: none
- abnormal responses: none
- reference substances: positive control of animals conducted with chloroacetamide; LC 50 after 48 hours: about 26 mg/L (corresponds to normal sensitivity)