JAUNE REACTIF 181

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 June 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

1984

Deviations:

yes

Remarks:

- Sludge from a Husman laboratory apparatus

Qualifier:

according to guideline

Guideline:

other: ETAD method No. 103

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test substance: FAT-40'400/A
Stability: guaranteed by the sponsor
Solubility: ~ 100 g/l
Storage: room temperature
Appearance: solid

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: Mixed culture of bacteria: Sludge of a Husman apparatus

Details on inoculum:

Laboratory culture: Mixed culture of bacteria: Sludge of a Husman apparatus.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

21.3°C

Nominal and measured concentrations:

Nominal concentrations: 1, 3.2, 10, 32 and 100 mg/L

Details on test conditions:

- Temperature: 21.3°C
- Duration: 3 h
- Estimation technique: Oxygen consumption measured with an oxygen sensitive electrode system: OXY DIGI 550.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The IC50 (3 h) of FAT-40'400/A is > 100 mg/l.

Results with reference substance (positive control):

The IC50 of the reference substance 3,5-Dichlorophenol is: 19 mg/L (Criteria: 10 - 25 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

IC50 for FAT 40400/A >100 mg/L.

Executive summary:

The IC50 of the test substance was determined in a respiration inhibition test performed according to OECD TEST Method 209, April 1984, corresponding to ETAD-Method No.103 under GLP. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 hours. The test substance had no significant inhibitory effect (<15%) on the respiration rate of activated sludge after the incubation period of 3 hours, on the test conditions. Based on the study results, IC50 was estimated to be >100 mg/L.

Description of key information

The IC50 of the test substance was determined in a respiration inhibition test performed according to OECD TEST Method 209, April 1984, corresponding to ETAD-Method No.103 under GLP. In this study, a mixed culture of bacteria (sludge of a Husman apparatus) was exposed to 1, 3.2, 10, 32 and 100 mg of test substance/L for 3 hours. The test substance had no significant inhibitory effect (<15 %) on the respiration rate of activated sludge after the incubation period of 3 hours, on the test conditions. Based on the study results, IC50 was estimated to be >100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L