JAUNE REACTIF 181

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

modified AFNOR-TEST

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 June 1990 - 24 July 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (old version) (Ready Biodegradability: Modified AFNOR Test)

Deviations:

yes

Remarks:

-Sterilization instead of steril filtration. - Determination of bacteria concentration: by plating. - Inoculum: effluent of a domestic sewage. treatment plant(see "any other information on materials and methods")

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test substance: FAT-40400/A
Stability: guaranteed by the sponsor
Solubility: ca. 100 g/l
Storage: room temperature
Appearance: solid

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

- Strain/Inoculation: Mixture of polyvalent bacteria collected on 26.06.90 (effluent of a domestic sewage treatment plant (ARA-Rhein)).
- Bacteria concentration: 1.0E+07/mL.

Duration of test (contact time):

28 d

Initial conc.:

40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

- Water/nutrient: according to guideline
- Temperature: 25 ± 1 °C
- Lighting: Indirect lighting
- Estimation technique: TOC/DOC SHIMADZU TOC-500

Reference substance:

other: glucose

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 0

Remarks on result:

other: sampling time not reported

Details on results:

Biodegradation corrected by the blank control and measured as DOC (mg/L) for the test substance is: 0 % (28th day)

Results with reference substance:

Biodegradation corrected by the blank control and measured as DOC (mg/L) for the reference substance (glucose) is: 100 % (28th day)

Validity criteria fulfilled:

yes

Remarks:

Difference of DOC values of replicates at the end of the test (28th day) is not more than 20 % and degradation of reference substance has reached the pass value within 14 days. Also the substance is not inhibitory (301 Guideline criteria, 1992).

Interpretation of results:

not readily biodegradable

Conclusions:

FAT 40400/A is not readily biodegradable (0% DOC) under the test conditions.

Executive summary:

In a ready biodegradability test performed according to OECD Guideline 301A (modified AFNOR test), biodegradation of the test substance by microorganisms was determined. In this study, a mixture of polyvalent bacteria (1.0E+07/mL) from an effluent of a domestic sewage treatment plant (ARA-Rhein) was exposed to 40 mg/L DOC of test substance for 28 days. After 28 days no biodegradation was observed. The results from inhibitory control showed that no inhibition of the activity of the bacteria can be found.

Biodegradation corrected by the blank control and measured as DOC (mg/L) for the reference substance (glucose) is: 100 % (28th day).

Description of key information

In a key study, the ready biodegradability of the test substance was determined in a GLP-compliant study according to OECD Guideline 301A (CG, 1990). The initial test substance concentration was 40 mg/L based on DOC. Biodegradation of the test substance after 28 days was found to be 0 %. No inhibition on the activity of the bacteria was observed in a inhibition control. The substance is to be considered not readily biodegradable. 

Biological oxygen demand over 5 days (BOD5) was determined in a study performed according to the EEC, Annex V, part C.8 guideline (D&C, 1988). In this study, a mixed culture of bacteria (effluent of a Husman laboratory apparatus) was exposed to 1, 5, 10, 20, 50, 100, 200 and 500 mg of test substance/L. The BOD5 of the test substance, corrected by the blank control, was 0 mg/g oxygen. The chemical oxygen demand (COD) of the test substance was determined in a study performed according to the EEC, Annex V, part C.9 guideline (D&C) under GLP. In this study, 5 mg of the test substance were exposed to 148 °C for 2 hours. The COD of the test substance was estimated 773 mg O2/g test item. The BOD5/COD ratio supports the conclusion that the test substance is not readily biodegradable. Reactive Yellow 181 was reported to be not readily biodegradable in a 2019 study conducted by MITI, Japan. The details about the study are not available.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater