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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
10 April 1990 to 12 April 1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to guideline
Guideline:
other: EPA- 540/9-85-005, June, 1985 (Acute Toxicity Test for Freshwater Invertebrates)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
BG 3247/TV 6
- Expiration date of the lot/batch:
March 1995

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
room temperature
- Stability under test conditions:
>72 h in Water
- Solubility of the test substance in water: >100 g/l
Analytical monitoring:
yes
Details on sampling:
Samples were taken at the beginning and the end of the testing. The samples were stored deep-frozen (-20° C) until the analysis was performed.
Vehicle:
no
Details on test solutions:
The test article was weighed and directly dissolved in the test water before introduction of Daphnia.
Test organisms (species):
Daphnia magna
Details on test organisms:
The study was performed with young Daphnia magna Strauss (6-24 hours old), bred in the laboratories of CCR under standardized conditions. The parental daphnids were maintained in the same reconstituted water as in the test for at least 24 hours prior to the start of the test.
.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
2.5 mmol/L as CaCO3
Test temperature:
18.2-18.4 °C
pH:
7.5-7.6
Dissolved oxygen:
8.7 - 9.0 mg/L
Nominal and measured concentrations:
- Nominal concentration: 1000 mg/L.
- Measured concentration: 138 % -145 % of nominal concentration.
Details on test conditions:
- In each test concentration and controls 20 daphnids were divided into four groups of five animals, each group in 50 mL test medium in a glass-beaker ( 100 ml). The test was performed in an air-conditioned room.
- Water-temperature: 18.2 - 18.4 °C
- Light: 16 hours/day, about 500 - 2000 Lux
- Reconstituted water aerated to saturation was used.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Only the nominal concentration of 1000 mg test article/L and a control was tested, because concentrations exceeding 1 g/L should not be tested according to the OECD Guideline No. 202 and in a range-finding test (not reported) with the nominal concentrations 1, 5, 25, 125 and 625 mg test article/L, the immobility rate of 20 daphnids per concentration was only 5 % or lower. The NOEC (highest concentration tested without toxic effect) of FAT 40400/A after 48 hours test duration is 1000 mg/L or higher. Therefore, the 48 h EC 50 could not be specified. In the control as well as test substance group no immobility or mortality was observed. During test duration the dissolved oxygen concentrations in the test media were 8.7 mg/L or higher, the pH in the range of 7.5 to 7.6. The test article concentration in the analysed test medium was 138- 145 % of the nominal value of 1000 mg/L.

Analytical results


The detection wave length of 420 nm was obtained by measuring the absorbance of FAT 40400/A in the wave length range from 280 to 700 nm in steps of 1.0 nm. The mean concentrations in g/1 and Z were calculated on the basis using the rounded results of the concentration in g/l. The spectrometer was adjusted to zero against water for standards and samples.

Validity criteria fulfilled:
yes
Conclusions:
The 48-h NOEC of FAT 40400/A is determined at >1000 mg/L.
Executive summary:

The acute toxicity to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline 202 and EU method C 2, and to the Guideline of the U.S. Environmental Protection Agency, Pesticide Assessment Guidelines, Subdivision E, EPA-540/9-85-005, June, 1985. The test was performed in compliance with the Good Laboratory Practice Regulations. Only the nominal concentration of 1000 mg testsubstance/L and a control was tested, because concentrations exceeding 1000 mg/L should not be tested according to the OECD Guideline No. 202. After 48 hours test duration no no immobility or mortality was observed. The reported result is related to the nominal concentration of the test article. During test duration the mean test article concentration was 142 % of the nominal value, the 48-h NOEC of FAT 40400/A is determined at >1000 mg/L, therefore, the 48 h EC50 could not be specified. So, under test conditions the test article was sufficiently stable.

Description of key information

The acute toxicity of the test substance to aquatic invertebrates was determined in a GLP-compliant test according to OECD 202. In this study, Daphnia magna (4 replicates of 5 daphnids each) were exposed to a nominal concentration of 1000 mg/L of the test substance for 48 hours (static) and a control. Analytically determined test item concentrations in the test medium ranged from 138 % at the start (hour-0) of the test to 145 % of nominal at the end (48 hours) of the test. No effect on mobility of Daphnia was noted throughout the test. Therefore, the 48-h NOEC value was determined at >1000 mg/L. Therefore, the 48 h EC50 could not be specified.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information