JAUNE REACTIF 181

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 July 1990 to 13 July 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

- Holding: in dechlorinated tap water - Test: in dechlorinated tap water - Temperature: higher temperature variation - Limit test: 2 concentrations tested

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

- Holding: in dechlorinated tap water - Test: in dechlorinated tap water - Temperature: higher temperature variation - Limit test: 2 concentrations tested

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test substance: FAT-40400/A
Stability: guaranteed by the sponsor
Solubility: ca. 100 g/l
Storage: room temperature
Appearance: solid

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the bath at the beginning and at the end of the experiment .

Vehicle:

no

Details on test solutions:

The test substance was disolved in 200 ml water, which was slowly added to the fish bath.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test organism: Zebrafish (Brachidanio rerio)
- Weight: 0.26 g
- Length: 30.0 mm
- Age: 112 days
- Acclimation: 26 days in dechlorinated tap water
- Supplier: West Aquarium, Bad Lauterberg, BRD

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.4 - 23.4 °C

pH:

7.7-8.3

Dissolved oxygen:

88-95 % of the air saturation value.

Nominal and measured concentrations:

- Nominal concentrations: 562, 1000 mg/L (based on results from pre-test).
- Measured concentration 562 mg/L: 105.9 -113.7 % of nominal.
- Measured concentration 1000 mg/L: 98.8 - 105.8 % of nominal.

Details on test conditions:

- Vessels 5 liter glass aquaria
- Volume : 2.9 litre (3.0 litre minus 2x0.05 litre for analytical control).
- Diluent: In dechlorinated tap water
- Mode of preparation: The test substance was disolved in 200 ml water, which was slowly added to the fish bath.
- Number of fish: 10 fish per concentration and water control
- Feeding: None
- Aeration: slowly aerated
- Lighting: 12 hours light I 12 hours darkness (yellow light TL 16/20 W)
- Measurements: at start and each day

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

There were no mortalities were seen during the experimental period. There were no abnormal responses of the fish were observed.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of FAT 40400/A is determined at >1000 mg/L in Zebra fish.

Executive summary:

Acute toxicity to fish of the test substance was determined in a study performed according to the OECD guideline 203 and the EU method C.1. Deviations from the revised OECD 203 Guideline were observed in regard to the number of limit tests (two were conducted in different concentrations) and the excess of the 100 mg/L limit concentration of test subsance. Pretests were conducted for 100, 300 and 1000 ppm concentrations and no mortalities or abnormalities occured. For the testings, ten zebrafish per concentration were placed in a slowly aerated static system and exposed to 562 ppm (or 562 mg/L) and 1000 ppm (or 1000 mg/L) of the test substance and in a blank control solution for 96 hours. Water samples were taken from the fish tank at the beginning and at the end of the experiment to determine concentrations of the test substance present. No mortalities or abnormalities of the fish were observed in the test and control solutions. Based on the study results, the LC50 of FAT 40400/A is determined at >1000 mg/L in Zebra fish.

Description of key information

The acute toxicity of the test substance to fish was determined in a GLP-compliant test according to the OECD Guideline 203. In this study, zebrafish (B. rerio; 10 per concentration) were exposed to nominal concentrations of 0, 562 and 1000 mg /L for 96 hours under static conditions. Measured concentrations ranged from 98.8 % to 105.9 % of nominal at the start (hour-0) of the test and from 105.8-113.7 % of nominal at the end (96 hours) of the test. During the test no abnormal responses in fish were observed and no deaths occurred. Based on these findings the 96-hour LC50 value was determined at >1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L