Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-445-8 | CAS number: 95-73-8
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-03-17 to 1989-04-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
Test material
- Reference substance name:
- Benzene, 2,4-dichloro-1-methyl-
- IUPAC Name:
- Benzene, 2,4-dichloro-1-methyl-
- Reference substance name:
- 2,4-dichlorotoluene
- EC Number:
- 202-445-8
- EC Name:
- 2,4-dichlorotoluene
- Cas Number:
- 95-73-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 2,4-dichloro-1-methylbenzene
- Details on test material:
- Content: 99,4 %
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Test animals:
- Strain: Wistar rats.
- Source: Winkelmann, Borchen
- Sex: males and females
- Age: 2 - 3 month at the beginning of the test.
- Weight: Average weight at the beginning of the test: 180 - 190 g
Conditions:
- Temperature: 22 +/- 2°C
- Photoperiod: 12 h light / dark cycle
- Humidity: about 50 %
- Diet: Altromin R 1324 and tap water ad libitum.
- Housing: 5 animals per cage
- Acclimation period: at least 5 days
- Air changes (per hr): about 10 times per hour
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- clean air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: plexiglass tubes
- Method of holding animals in test chamber: exposure tubes were locked on one end in a way that the tail was outside of the tube
- Method of conditioning air: generation of compressed air and automatically conditioning
TEST ATMOSPHERE
- Assay of 2,4-dichlorotoluene in atmosphere: gas chromatography with FI-detector - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- gas chromatography with FI-detector
- Duration of exposure:
- 4 h
- Concentrations:
- 0 mg/m³, 1270 mg/m³, 2669 mg/m³
- No. of animals per sex per dose:
- 5 animals per sex per dose in the treatment groups and 10 animals per sex in the control group
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: not clearly stated
- Frequency of weighing: before exposure, day 3, 7 and 14 post exposure
- Necropsy of survivors performed: yes
- Other examinations performed: clinical observations with special regard to the mucous membranes of the eyes and respiratory tract, general condition, respiration, cardiovascular system, behaviour, central-nervous disturbances, reflex-activity
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 2 669 mg/m³ air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- no animals died during exposure or post exposure period
- Clinical signs:
- other: No clinical symptoms were recorded in animals of the control and tested group.
- Body weight:
- not affected
- Gross pathology:
- no pathological findings
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LC50 value for 2,4-dichlorotoluene was determined to be > 2669 mg/m³.
- Executive summary:
Acute inhalation toxicity of 2,4-dichlorotoluene has been investigated with male and female Wistar rats which were exposed for 4 hours towards 0, 1270 and 2669 mg/m³ (analytically concentrations).
Exposure to all concentrations of 2,4-dichlorotoluene was tolerated by all animals without any symptoms of intoxication.
No macroscopically visible changes occurred in the lungs or in any other tissue and organ.
No sex specific differences were observed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
