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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report date:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Benzene, 2,4-dichloro-1-methyl-
IUPAC Name:
Benzene, 2,4-dichloro-1-methyl-
Constituent 2
Chemical structure
Reference substance name:
2,4-dichlorotoluene
EC Number:
202-445-8
EC Name:
2,4-dichlorotoluene
Cas Number:
95-73-8
Molecular formula:
C7H6Cl2
IUPAC Name:
2,4-dichloro-1-methylbenzene
Details on test material:
State of matter: liquid
colour: colourless
content: > 99 %
Containments: 4-chlorotoluene; 3,4-dichlorotoluene and other isomers of dichlorotoluene

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Hoechst AG, Kastengrund, Germany
- Average weight at study initiation: 1.8 - 2.1 kg

ENVIRONMENTAL CONDITIONS
- Temperature: 20 +/- 3 °C
- Humidity: 50 +/- 20 %
- Photoperiod: 12 h dark, 12 h light
- Housing: one animal per cage
- Diet: "Altromin 2123" ad libitum and 15 g hay per day
- Water: tap water (ad libitum)

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
0.5 mL 2,4-dichlorotoluene (pur).
Duration of treatment / exposure:
4 h
Observation period:
14 days
Number of animals:
3
Details on study design:
TEST SITE
- the skin was shaved 24 h bevore test. Only animals with unimpaired skin were used.
- Area of exposure: 2.5 x 2.5 cm.
- Coverage: plaster with a layer of cellulose.

After 4 hours of exposure the skin was washed with water.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
edema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0
Max. score:
4
Irritation parameter:
edema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0
Max. score:
4
Irritation parameter:
edema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
0.3
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
24/48/72 h
Score:
2.7
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
fully reversible

Applicant's summary and conclusion

Conclusions:
2,4-Dichlorotoluene is classified (67/548/EEC) as skin irritant, symbol Xi, R38.
Executive summary:

In a dermal irritation study (Hoechst AG, 1986) 3 New Zealand White rabbits were dermally exposed to 2,4-dichlorotoluene (pure). The test site (intact, shaved skin) was covered with a plaster with a layer of cellulose for 4 hours. The skin then was washed with water. Rabbits were observed for 14 days. Irritations regarding erythema and edema were scored after 30-60 min, 24 hours, 48 hours, 72 hours, 7 days and 14 days.

The test results lead to a classification according to OECD Guideline 404: skin irritant, symbol Xi, R38.