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Description of key information

In the key study the NOEC of the test item is 1000 mg/L, the EC50 of the test item is > 1000 mg/L.
The test item is not toxic up to the concentration of 1000 mg/L, to activated sludge of a municipal sewage treatment plant. 
This is supported by a second study with the gegradation product 8:2 FTOH. This substance is also not toxic in concentrations <1000 mg/L to activated sludge of a municipal sewage treatment plant.

Key value for chemical safety assessment

Additional information

Key study:

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item from 2013-01-07 to 2013-01-08, with the definitive experimental phase on 2013-01-08 at theDr.U.Noack-Laboratorien, 31157Sarstedt, Germany.Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the substance concentrations of 500 and 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates were -6 % and 3 %.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 97.9 mg/L.

NOEC and EC-Values of Fluowet AC 812 solid

EC-values
[mg/L]

NOEC*

1000

EC20

> 1000

EC50

> 1000

EC80

> 1000

                        *) No statistically significant effect (p 0.05), ANOVA

The NOEC of the test itemFluowet AC 812 solid is1000mg/L,
the EC50of the test item is > 1000 mg/L.

The test item is not toxic up to the concentration of1000mg/L to activated sludge
of a municipal sewage treatment plant.

Supporting study:

A Respiration lnhibition Test with activated sludge according to OECD Guideline No. 209 and GLP principles was performed for 3 hrs with the test substance. The test system was activated sludge of the municipal treatment plant. The test method was static. Nominal concentrations of the test item were as follows:100, 180, 320, 580, and 1000 mg/L. The respiration rates of control, reference and test item were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. The inhibition ranged from 2 to 9 %. The EC20, EC50 and EC80 values could not be calculated in the tested concentration range. In order to check the activity of the test system and the test conditions a reference test is carried out once every 7 days with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity is determined. The EC50 value for the reference item was 104 mg/L.

Validity criteria of the test guideline were fulfilled.

The test substance is not toxic in concentrations < 1000 mg/L to activated sludge of a municipal sewage treatment plant.