2-Propenoic acid, γ-ω-perfluoro-C8-14-alkyl esters

Toxicity to aquatic algae and cyanobacteria

Description of key information

In this key study the test item caused slight inhibitions on growth of the freshwater green alga Desmodesmus subspicatus after 72 hours exposure when tested with the limit concentration of 1.30 mg/L.

The nominal NOEC for inhibition of growth rate and yield after 72 hours was < 1.30 mg/L. The nominal EC50-values for inhibition of growth rate and yield were > 1.30 mg/L. For further EC-values. All effect levels are given based on the nominal concentration of Fluowet AC 812 solid, which was analytically verified via GC-MS.

Both supporting studies show that there is no toxic effects up to the water solubility.

Additional information

Key study:

The toxicity of the test itemto the unicellular freshwater green algaDesmodesmus subspicatuswas determined according to the principles of OECD 201atDr.U.Noack-Laboratorienin 31157 Sarstedt, Germanyfrom 2013-01-22 to 2013-02-16, with the definite exposure phase from2013-02-12 to 2013-02-15.The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours in a limit test.

The study was conducted under static conditions with an initial cell density of 4332 cells/mL.Based on the results of a preliminary range finding test the limit concentration of 1.30 mg/L was tested, using methanol as solvent. Therefore, a solvent control containing dilution water spiked with 0.100 mL Methanol/L was tested additionally. The nominal loading of 1.30 mg/L corresponds to the maximum solubility level of the main component C8-Perfluoroalkyl-ethylacrylate (water solubility: 0.7 mg/L, nominal content: 54.0 %). Six replicates were tested for the limit concentration, the solvent control and the control, respectively.With regard to the volatility of the test item, glass flasks without headspace were used to reduce the losses of the test item via the surface. Environmental conditionswere within the acceptable limits.

The limit concentration, the solvent control and the control were analysed by GC-MS after 0 hours (fresh media) and 72 hours (old media). The measured concentration of the main component C8-Perfluoroalkylethylacrylate of FluowetAC 812 solid(content 54.0 %) at test startwas 100 %of the nominal value. At the end of the testthe main componentgave measured concentration of 87 % of the nominal value. For details of the analytical results. All effect values are given based on the nominal concentration of the test item.

NOEC, LOEC, EC - Values of FluowetAC 812 solid(0 - 72 hours)

 based on nominal test item concentration [mg/L]

	Growth Rate Inhibition
NOEC	1.30
LOEC	1.30
ErC10	1.30
ErC20	> 1.30
ErC50	> 1.30
	Inhibition of Yield
NOEC	1.30
LOEC	1.30
EyC10	1.30
EyC20	1.30
EyC50	> 1.30

The study (SSO84111) was conducted under static conditions over a duration of 72 hours with an initial cell density of 10⁴cells/mL and a saturated solution as limit concentration, containing 0.20 mg/L. For the preparation of the saturated solution a suspension with a nominal loading of 100 mg/L was shaken for 96 hours and centrifuged. The clear supernatant was used for the test.

The test vessels were coated with the test item for 14 days before the test started. Three replicates were tested for the limit concentration and six replicates for the control. The test item was clearly dissolved throughout the test. Microscopic evaluation of the cells at study start and end of the incubation period revealed no morphological abnormalities. Water quality parameters of pH-value, measured at 0 and 72 hours, and room temperature, measured continuously, were deemed to be within acceptable limits.

The initial measured concentration of the saturated solution was 0.20 mg/L. At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass walls of the test vessels was unlikely as the walls were already pre-coated for 14 days prior to study start.

The 72-h EbC50 (inhibition of biomass growth) > 0.20 mg/L.

The 72-h ErC50 (rate related inhibition) > 0.20 mg/L.

The 72-h NOEC for both endpoints = 0.20 mg/L.

The 72-h LOEC for both endpoints was > 0.20 mg/L.

Supporting studies:

The study (SSO84112) was conducted under static conditions over a duration of 72 hours with an initial cell density of 10⁴cells/mL and a water accomodated fraction as limit concentration, containing 0.47 mg/L. For the preparation of the water accomodated fraction a suspension with a nominal loading of 100 mg/L was shaken for 96 hours.

The test vessels were coated with the test item for 14 days before the test started. Three replicates were tested for the limit concentration and six replicates for the control. The test item was clearly dissolved throughout the test. Microscopic evaluation of the cells at study start and end of the incubation period revealed no morphological abnormalities. Water quality parameters of pH-value, measured at 0 and 72 hours, and room temperature, measured continuously, were deemed to be within acceptable limits.

The initial measured concentration of the saturated solution was 0.47 mg/L. At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass walls of the test vessels was unlikely as the walls were already pre-coated for 14 days prior to study start.

The 72-h EbC50 (inhibition of biomass growth) > 0.47 mg/L.

The 72-h ErC50 (rate related inhibition) > 0.47 mg/L.

The 72-h LOEC for both endpoints was > 0.47 mg/L.

The 72-h NOEC for both endpoints = 0.47 mg/L.