2-Propenoic acid, γ-ω-perfluoro-C8-14-alkyl esters

Short-term toxicity to fish

Description of key information

In the keystudy, the test item was found to cause no lethal effects to Zebrafish after 96 hours at a nominal test concentration of 1.30 mg/L.
The LC50 after 96 hours is > 1.30 mg/L.

All effect levels are given based on the nominal concentrations of the test item Fluowet AC 812 solid.
Also all supporting studies shows that there is no toxic effects up to the water solubility.

Additional information

Key study:

The acute toxicity of the test item to fish (zebrafish), was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) from 2013-01-21 to 2013-01-29 with the definitive exposure phase from 2013-01-21 to 2013-01-25, atDr.U.Noack-Laboratorien, 31157 Sarstedt, Germany.

A semi-static limit test with daily renewal of the test media was conducted with the nominal test item concentration of 1.30 mg/L. The nominal loading of 1.30 mg/L corresponds to the maximum solubility level of the main component 2-(Perfluorooctyl)ethyl acrylate (water solubility: 0.7 mg/L, nominal content: 54.0 %). The test was carried out without headspace. Duration of the test was 96 hours. 7 test organisms were exposed to the limit test concentration and the solvent control (without test item) containing the same solvent concentration as the test replicate. Water quality parameters temperature, pH-value and O2-saturation measured at 0, 24, 48, 72 and 96 hours were within the acceptable limits.

The determination of the concentration of 2-(Perfluorooctyl)ethyl acrylate (main component of thethe test itemFluowet AC 812 solid) was carried out via GC-MSfrom freshly prepared media after 0 and 72 h, and from the corresponding 24 h old test media after 24 and 96 h. The measured concentrations ofFluowet AC 812 solid(determined via content of 2-(Perfluorooctyl)ethyl acrylate) in freshly prepared media were 102 % of the nominal value and 83 – 90 % in 24 h aged test media. All effect levels are given based on the nominal concentrations of the test itemFluowet AC 812 solid.

LC-Valueswith 95 % Confidence Intervals (0 – 96 hours)

                  Based on nominal test item concentrations [mg/L]

Test duration [h]	LC50	p = 95 %
24	> 1.30	n.a.
48	> 1.30	n.a.
72	> 1.30	n.a.
96	> 1.30	n.a.
LC100=	> 1.30
Lowest test item concentration
with 100 % mortality after 96 h
LC0=	1.30
Highest test item concentration
with 0 % mortality after 96 h

A static limit test (FAZ84111) was conducted with a saturated solution. The test vessel was coated with the test item for 14 days before the test started. Duration of the test was 96 hours. Thirty test organisms were exposed to the saturated solution and the control. For the preparation of the saturated solution a suspension with a nominal loading of 100 mg/L was shaken for 96 hours and centrifuged. The clear supernatant was used for the test.

All effect levels are based on the initially measured concentration of the test item (0.18 mg/L). At the end of the test the concentration of the test item was 0.0063 mg/L. Sorption to the glass of the test vessels is unlikely as the walls were already pre-coated for 14 days before the test started.

The 96-hour NOEC and LC0 was 0.18 mg/L. The 96-hour LC50 and LC100 was > 0.18 mg/L.

Supporting studies:

A static limit test (FAZ84112) was conducted with the water accomodated fraction. The test vessel was coated with the test item for 14 days before the test started. Duration of the test was 96 hours. Thirty test organisms were exposed to the water accomodated fraction and the control. For the preparation of the water accomodated fraction, a suspension with a nominal loading of 100 mg/L was shaken for 96 hours. After phase separation, the clear liquid phase was used for the test.

All effect levels are based on the initially measured concentration of the test item (0.14 mg/L). At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass of the test vessels is unlikely as the walls were already pre-coated for 14 days before the test started.

The 96-hour NOEC and LC0 was 0.14 mg/L. The 96-hour LC50 and LC100 was > 0.14 mg/L.