2-(hydroxymethylamino)ethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Data from study report

Qualifier:

according to guideline

Guideline:

other: As per mentioned below

Principles of method if other than guideline:

EPA Pesticide Assessment Guidelines FIFRA Subdivision E, Hazard Evaluation: Wildlife and Aquatic Organisms, EPA 540/9-82-024, Oct. 1982 and ASTM Standard E 729-88, Standard Practice for Conducting Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2-(hydroxymethylamino)ethanol
- Molecular formula (if other than submission substance): C3H9NO2
- Molecular weight (if other than submission substance): 91.1091 g/mol
- Smiles notation (if other than submission substance): CN(CCO)O
- InChl (if other than submission substance): 1S/C3H9NO2/c1-4(6)2-3-5/h5-6H,2-3H2,1H3
- Substance type: Organic
- Physical state: Liquid

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: No data available
- Age at study initiation (mean and range, SD): Juveniles
- Length at study initiation (length definition, mean, range and SD): Mean - 44 mm, Range 39 to 48 mm
- Weight at study initiation (mean and range, SD): wet weight: Mean - 0.72 g, Range 0.39 to 1.0 g


ACCLIMATION
No data available

QUARANTINE (wild caught)
No data available

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

No data available

Test temperature:

20±1°C

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

TEST SYSTEM
- Test vessel: A continuous-flow diluter
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: Test chambers were Teflon®-lined, 25-L polyethylene aquaria filled with 15 L of test water. The depth of the test water in each chamber was approximately 17 cm.
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available

TEST MEDIUM / WATER PARAMETERS
No data available

OTHER TEST CONDITIONS
No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available

TEST CONCENTRATIONS
No data available

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: visual examination of the mortality, and clinical observation data

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

The pattern of mortality in this study did not allow for calculating an LC50 value; therefore, an estimation of the LC50 value was made by a visual inspection of the mortality data.

Validity criteria fulfilled:

not specified

Conclusions:

The 96-hour LC50 value for rainbow trout exposed to Troysan 174 was >100 mg/L. The 96-hour no observed effect concentration was 100 mg/L, as determined by visual examination of the toxicity data.

Executive summary:

Rainbow trout were exposed to a five test concentrations of Troysan 174 and a negative (well water) control for 96 h. Two replicate test chambers were maintained in each treatment and control group, with 10 fish in each test chamber. Nominal test concentrations selected were 13.0, 21.6, 36.0, 60.0, and 100 mg of Troysan 174/L which is based upon the results of acute range finding toxicity tests. Observations of mortality and other clinical signs of toxicity were made at 2, 24, 48, 72, and 96 hours. The 96-hour LC50 value for rainbow trout exposed to Troysan 174 was >100 mg/L. The 96-hour no observed effect concentration was 100 mg/L, as determined by visual examination of the toxicity data. 

Description of key information

Rainbow trout were exposed to a five test concentrations of Troysan 174 and a negative (well water) control for 96 h. Two replicate test chambers were maintained in each treatment and control group, with 10 fish in each test chamber. Nominal test concentrations selected were 13.0, 21.6, 36.0, 60.0, and 100 mg of Troysan 174/L which is based upon the results of acute range finding toxicity tests. Observations of mortality and other clinical signs of toxicity were made at 2, 24, 48, 72, and 96 hours. The 96-hour LC50 value for rainbow trout exposed to Troysan 174 was >100 mg/L. The 96-hour no observed effect concentration was 100 mg/L, as determined by visual examination of the toxicity data. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Four results including different experimental and predicted data from validated models for short term fish toxicity endpoint of target chemical 2-[hydroxy(methyl)amino]ethanol (Cas no. 34375-28-5) were summarized as follows:

 

First study from HPVIS database by US EPA; 2017 which indicate Rainbow trout were exposed to a five test concentrations of Troysan 174 and a negative (well water) control for 96 h. Two replicate test chambers were maintained in each treatment and control group, with 10 fish in each test chamber. Nominal test concentrations selected were 13.0, 21.6, 36.0, 60.0, and 100 mg of Troysan 174/L which is based upon the results of acute range finding toxicity tests. Observations of mortality and other clinical signs of toxicity were made at 2, 24, 48, 72, and 96 hours. The 96-hour LC50 value for rainbow trout exposed to Troysan 174 was >100 mg/L. The 96-hour no observed effect concentration was 100 mg/L, as determined by visual examination of the toxicity data. 

Above aquatic classification supported by ECOTOX database,2017 suggest In Acute fish toxicity study, the lethal concentration (LC50) was determined for test substance 2-[hydroxy(methyl)amino]ethanol with an exposure period of 96 hrs. on the basis of effects on mortality. Test organism used was Oncorhynchus mykiss in static freshwater system. The Lethal concentration to 50% of test organisms(LC50) on the basis of Mortality effect was observed to be 0.0603 mg/l and No-observable-effect-level (NOEL) was observed to be 0.0307 mg/l for exposure period of 96 hrs.

Thus based on the above value, it is concluded that the test substance 2-[hydroxy(methyl)amino]ethanol hazarous to aquatic environment,since the substance is readily biodegradable in nature it is consider that test substance 2-[hydroxy(methyl)amino]ethanol not classified as per the CLP criteria.

And Prediction is done using OECD QSAR Toolbox version 3.3 with logKow as the primary descriptor data indicate the prediction was based on the effects of the test compound on Danio rerio (previous name: Brachydanio rerio) in a static fresh water system during a 96 hour exposure. The lethal concentration (LC50) for the test compound 2-[hydroxy(methyl)amino]ethanol (CAS no. 34375-28-5) was estimated to be 312.69 mg/l on the basis of mortality effect. Thus, based on this value, test chemical compound 2-[hydroxy(methyl)amino]ethanol was considered to be non toxic to fish and thus can be not classified as per the CLP regulations.

Above all results assist by prediction using the EPI Suite ECOSAR version 1.10, the short term toxicity on fish was predicted for test substance 2-[hydroxy(methyl)amino]ethanol (CAS: 34375-28-5). On the basis of effects observed in a static freshwater system, the lethal concentration LC50 value for the substance is estimated to be 447000 mg/l for fish for 96 h duration. Based on this value, it can be concluded that the test chemical 2-[hydroxy(methyl)amino]ethanol can be considered as non toxic to fish at environmentally relevant concentrations and can be considered to be not classified as per the CLP classification criteria.

Thus all available studies for short term fish toxicity endpoint come to conclusion that the test substance 2-[hydroxy(methyl)amino]ethanol (Cas no. 34375-28-5) is expected to be non toxic to fish and thus not consider for aquatic classification.