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Environmental fate & pathways

Hydrolysis

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Justification for type of information:
Data is from secondary source
Qualifier:
according to guideline
Guideline:
other: Study conducted in compliance with EPA GLP standards (40CFr 160 and according to EPA Pesticide Guidelines Subdivision N 161-1)
Principles of method if other than guideline:
Determination of aqueous hydrolysis of test chemical 2-(hydroxymethylamino)ethanol was carried out for 30 days at pH 5, 7 and 9, respectively.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: 2-(hydroxymethylamino)ethanol
- Common name: Troysan 174
- Molecular formula (if other than submission substance): C3H9NO2
- Molecular weight (if other than submission substance): 91.1091 g/mol
- Smiles notation (if other than submission substance): CN(CCO)O
- InChl (if other than submission substance): 1S/C3H9NO2/c1-4(6)2-3-5/h5-6H,2-3H2,1H3
- Substance type: Organic
- Physical state: Liquid
- Purity: 98.7%
Radiolabelling:
not specified
Analytical monitoring:
yes
Details on sampling:
Details on sampling
- Sampling intervals for the parent/transformation products: The solutions were sampled for immediate chromatographic analysis at intervals of 0, 1, 3, 7, 14, 21 and 30 days. Liquid chromatographic analysis of the fresh samples using post-column detection allowed quantitation of primary amines.
- Sample storage conditions before analysis: Samples from each time point were also placed in the freezer for further analysis.
Buffers:
Buffers
pH: 5, 7 and 9

Estimation method (if used):
Mean and standard deviation were calculated, and a linear regressions analysis was performed.
Details on test conditions:
TEST SYSTEM
- Sterilisation method: Efforts were made to maintain sterility [but no mention on method employed]
- Lighting: For the duration of the study, the solutions were maintained in darkness in a water bath adjusted to 25°C

TEST MEDIUM
- Volume used/treatment: The initial amount of test material dissolved in the buffers was 250µg/ml.
- Preparation of test medium: The buffers were autoclaved after preparation and before the test substance was added.

OTHER TEST CONDITIONS
- The temperature of the bath was recorded once daily, with an average of 25°C, ranging from 24.9 to 25.1°C.
Duration:
30 d
Temp.:
25 °C
Initial conc. measured:
250 mg/L
Number of replicates:
A validation study was undertaken, using buffer samples fortified with known amounts of Troysan 174 on the day of testing. Each set consisted of 3 replicate sample of Troysan 174 (at 0, 125 and 250 μg/ml) in each buffer (pH 5.0, 7.0 and 9.0).
Positive controls:
not specified
Negative controls:
not specified
Test performance:
Yes
Breakdown products: Formaldehyde and ethanolamine
Transformation products:
yes
No.:
#1
No.:
#2
% Recovery:
91.5
pH:
5
Temp.:
25 °C
Duration:
30 d
Remarks on result:
other: Other details not known
% Recovery:
97.5
pH:
7
Temp.:
25 °C
Duration:
30 d
Remarks on result:
other: Other details not known
% Recovery:
97.1
pH:
9
Temp.:
25 °C
Duration:
30 d
Remarks on result:
other: Other details not known
Key result
Remarks on result:
not measured/tested
Details on results:
The hydrolysis of Troysan 174 to formaldehyde and ethanolamine was too rapid to be quantified.
pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes/No : Yes

MAJOR TRANSFORMATION PRODUCTS
Formaldehyde and ethanolamine
Validity criteria fulfilled:
yes
Conclusions:
Aqueous hydrolysis of test chemical 2-(hydroxymethylamino)ethanol (CAS no. 34375 -28 -5) was carried out for 30 days at pH 5, 7 and 9, respectively. There was immediate, or nearly immediate hydrolysis of Troysan at pH 5.0, 7.0 and 9.0 at 25°C. The hydrolysis of Troysan 174 to formaldehyde and ethanolamine was too rapid to be quantified. The recovery of 2 degradation substances together after 30 days (calculated as a percentage of the initial, using Troysan 174 dissolved in water as the standard) averaged 91.5% at pH 5, 97.5% at pH 7 and 97.1% at pH 9, respectively. Formaldehyde and ethanolamine was the breakdown product of the test chemical 2-(hydroxymethylamino)ethanol.
Executive summary:

Aqueous hydrolysis of test chemical 2-(hydroxymethylamino)ethanol (CAS no. 34375 -28 -5) was carried out for 30 days at pH 5, 7 and 9, respectively.

Since radioactively labeled material was not available for the study, the 95% grade compound was used and chemical analysis was used to identify the breakdown products. The initial amount of test material dissolved in the buffers was 250 µg/ml. The buffers were autoclaved after preparation and before the test substance was added. Efforts were made to maintain sterility. For the duration of the study, the solutions were maintained in darkness in a water bath adjusted to 25°C. The temperature of the bath was recorded once daily, with anaverage of 25°C, ranging from 24.9 to 25.1°C. The solutions were sampled for immediate chromatographic analysis at intervals of 0, 1, 3, 7, 14, 21 and 30 days. Samples from each time point were also placed in the freezer for further analysis. Liquid chromatographic analysis of the fresh samples using post-column detection allowed quantitation of primary amines. There was immediate, or nearly immediate hydrolysis of Troysan at pH 5.0, 7.0 and 9.0 at 25°C. The hydrolysis of Troysan 174 to formaldehyde and ethanolamine was too rapid to be quantified. The recovery of 2 degradation substances together after 30 days (calculated as a percentage of the initial, using Troysan 174 dissolved in water as the standard) averaged 91.5% at pH 5, 97.5% at pH 7 and 97.1% at pH 9, respectively. Formaldehyde and ethanolamine was the breakdown product of the test chemical 2 -(hydroxymethylamino)ethanol.

Description of key information

Aqueous hydrolysis of test chemical 2-(hydroxymethylamino)ethanol (CAS no. 34375 -28 -5) was carried out for 30 days at pH 5, 7 and 9, respectively (HPV Chemical Challenge Program, 2016). Since radioactively labeled material was not available for the study, the 95% grade compound was used and chemical analysis was used to identify the breakdown products. The initial amount of test material dissolved in the buffers was 250 µg/ml. The buffers were autoclaved after preparation and before the test substance was added. Efforts were made to maintain sterility. For the duration of the study, the solutions were maintained in darkness in a water bath adjusted to 25°C. The temperature of the bath was recorded once daily, with an average of 25°C, ranging from 24.9 to 25.1°C. The solutions were sampled for immediate chromatographic analysis at intervals of 0, 1, 3, 7, 14, 21 and 30 days. Samples from each time point were also placed in the freezer for further analysis. Liquid chromatographic analysis of the fresh samples using post-column detection allowed quantitation of primary amines. There was immediate, or nearly immediate hydrolysis of Troysan at pH 5.0, 7.0 and 9.0 at 25°C. The hydrolysis of Troysan 174 to formaldehyde and ethanolamine was too rapid to be quantified. The recovery of 2 degradation substances together after 30 days (calculated as a percentage of the initial, using Troysan 174 dissolved in water as the standard) averaged 91.5% at pH 5, 97.5% at pH 7 and 97.1% at pH 9, respectively. Formaldehyde and ethanolamine was the breakdown product of the test chemical 2 -(hydroxymethylamino)ethanol.

Key value for chemical safety assessment

Additional information

Aqueous hydrolysis of test chemical 2-(hydroxymethylamino)ethanol (CAS no. 34375 -28 -5) was carried out for 30 days at pH 5, 7 and 9, respectively (HPV Chemical Challenge Program, 2016). Since radioactively labeled material was not available for the study, the 95% grade compound was used and chemical analysis was used to identify the breakdown products. The initial amount of test material dissolved in the buffers was 250 µg/ml. The buffers were autoclaved after preparation and before the test substance was added. Efforts were made to maintain sterility. For the duration of the study, the solutions were maintained in darkness in a water bath adjusted to 25°C. The temperature of the bath was recorded once daily, with an average of 25°C, ranging from 24.9 to 25.1°C. The solutions were sampled for immediate chromatographic analysis at intervals of 0, 1, 3, 7, 14, 21 and 30 days. Samples from each time point were also placed in the freezer for further analysis. Liquid chromatographic analysis of the fresh samples using post-column detection allowed quantitation of primary amines. There was immediate, or nearly immediate hydrolysis of Troysan at pH 5.0, 7.0 and 9.0 at 25°C. The hydrolysis of Troysan 174 to formaldehyde and ethanolamine was too rapid to be quantified. The recovery of 2 degradation substances together after 30 days (calculated as a percentage of the initial, using Troysan 174 dissolved in water as the standard) averaged 91.5% at pH 5, 97.5% at pH 7 and 97.1% at pH 9, respectively. Formaldehyde and ethanolamine was the breakdown product of the test chemical 2 -(hydroxymethylamino)ethanol.