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EC number: 253-455-4 | CAS number: 37310-83-1
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Under the conditions of the performed Activated Sludge Respiration Inhibition Test, the EC50 value of test item was determined as higher than 100 mg/L. Based on the statistical evaluation in this test the NOEC was 19 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 318.56 mg/L
- EC10 or NOEC for microorganisms:
- 19 mg/L
Additional information
The purpose of the 3-hour test was to evaluate the influence of the test item Reaction product of oleylalcohol with polyphosphoric acid on the activity of the activated sludge by measuring the respiration rate under defined conditions. The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours.
Based on the preliminary information about the effects of the test item on the activated sludge inoculum, Reaction product of oleylalcohol with polyphosphoric acid was investigated at the nominal concentrations of 6; 19; 60; 190 and 600 mg/L. Defined amounts of the test item were added directly into the test vessels. 2, 7 and 24.5 mg 3,5-Dichlorophenol/L as positive reference control, furthermore blank (inoculum) control and nitrification controls were investigated in parallel. The main test was performed without abiotic controls, based on the results of the preliminary test where abiotic controls were tested at the test item concentration of 1000 mg/L and no remarkable abiotic oxygen consumption was noticed. The test was performed without pH adjustment. All validity criteria of the study were met.
The observed-calculated specific respiration rates remained within the range of the specific respiration rates of the blank controls at the concentrations of 6 and 19 mg/L. The inhibition of the specific respiration rates compared to the respiration rates of the blank controls showed clear dose-related tendency; not significant 4.44 % inhibition was obtained at 19 mg/L furthermore 10.82 %, 34.96 %, and 69.31 % at the concentrations of 60, 190 and 600 mg/L.
Based on measured inhibition rates the 3-hour EC10, EC50 and EC80 and their 95 %-confidence limits were calculated by Probit analysis using TOXSTAT software.
EC10: 52.36 mg/L (38.61-70.99)
EC50: 318.56 mg/L (251.14-404.07)
EC80: 1042.80 mg/L (706.13-1539.99)
The EC50 value was determined as 318.56 mg/L, consequently: EC50 > 100 mg/L.
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