Tetrasodium [2-({4-fluoro-6-[(2-{[4-fluoro-6-({5-(hydroxykappaO)-6-[(2-{[2-(hydroxy-kappaO)-5-sulfophenyl] diazenyl-kappaN1}-4,5-dimethoxyphenyl) diazenyl-kappaN2]-7-sulfo-2-naphthyl} amino)-1,3,5-triazin-2-yl] amino} propyl) amino]-1,3,5-triazin-2-yl} amino) benzene-1,4- disulfonato(6-)] cuprate(4-)

Endpoint summary

Description of key information

Study reports have been available for the ready biodegradability test, inherent biodegradability test, hydrolysis as a function of pH and adsorption/desorption test. The results are summarized as below:

The test item FAT 40825/A was investigated for its ready biodegradability in a manometric respirometry test over 28 days according to OECD Guideline for Testing of Chemicals No. 301 F (1992). The biochemical oxygen demand (BOD) of the test item FAT 40825/A in the test media was in the range of the inoculum controls throughout the entire 28-day exposure period. Consequently, FAT 40825/A was not readily biodegradable under the test conditions within 28 days. The test item FAT 40825/A was investigated for its potential (inherent) ultimate biodegradability in a Zahn-Wellens / EMPA test over 28 days, based on the OECD Guideline for Testing of Chemicals, No. 302 B (1992). In the test flasks, containing the test item and activated sludge (inoculum), the mean concentration of DOC (dissolved organic carbon) varied between 94 and 101 mg/L over the 28-day exposure period, and was not significantly different from the initial mean DOC concentration of 101 mg/L, measured on Day 0 (starting value after 3 hours of exposure). Expressed as percentage DOC-removal, mean values in the range from 1 to 7 % were noted. FAT 40825/A was not biodegradable under the test conditions within 28 days. The hydrolysis of the test item was performed under GLP compliance at pH 4.0, pH 7.0 and pH 9.0. The result showed FAT 40825/A at pH 4.0 has a half-life time of 68 hours (3 days) at 25 °C, is stable at pH 7.0 and its half-life time is longer than one year at 25 °C, and at pH 9.0 has a half-life time of 3576 hours (149 days) at 25 °C. The determination of the adsorption coefficient (Koc) of FAT 40825/A was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001. The log of the adsorption coefficient (Koc) of FAT 40825/A was estimated to be log Koc <1.25 which is equal to a Koc value of <18 using the HPLC method. This value indicates that FAT 40825/A will not be adsorbed by organic carbon in soil.