[No public or meaningful name is available]

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6.9.2019 - 20.10.2017

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

version 2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

1,1,3,3-tetrachloroprop-1-ene

EC Number:

675-808-5

Cas Number:

18611-43-3

Molecular formula:

C3H2Cl4

IUPAC Name:

1,1,3,3-tetrachloroprop-1-ene

Test material form:

liquid

Details on test material:

concentration 99,87%

Test animals

Species:

rat

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Rationale for alternative/additional species to rat (if applicable)
- Source:
- Females (if applicable) nulliparous and non-pregnant: yes
- Rationale for use of males (if applicable)
- Age at study initiation: 8 weeks
- Weight at study initiation: 150g
- Fasting period before study: 20hours
- Housing: plastic breeding cage
- Historical data:
- Diet (e.g. ad libitum): pelleted diet for rats-Alltromin
- Water (e.g. ad libitum):yes, ad libitum
- Acclimation period:5 days
- Microbiological status when known
- Method of randomisation in assigning animals to test and control groups

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-3°C
- Humidity (%): 30-70%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: To:

Administration / exposure

Vehicle:

olive oil

Remarks:

Batch: 8002182001, expiration: 7/2018,

Details on oral exposure:

The test substance was administered to the stomach by tube. The single volume of administered suspension as 1 mL/100gof animal body weight. The starting dose was 300 mg/kg of body weight.

VEHICLE olive oil
- Concentration in vehicle: 300mg/kg of body weight
- Amount of vehicle (if gavage):
- Justification for choice of vehicle:
- Lot/batch no. (if required): 8002182001
- Purity:

MAXIMUM DOSE VOLUME APPLIED:

DOSAGE PREPARATION (if unusual):

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:

Doses:

Start 300 mg/kg,
step No.2: 2000 mg/kg
step No.3 300mg/kg

No. of animals per sex per dose:

3 females

Details on study design:

Step no. 1 -start 300 mg/kg body weight for 3 females- no deaths
step no.2 - 2000 mg/kg body weight for 3 females - daths of all animals
Step 3 300 mg/kg body weight for 3 females- no deaths

Statistics:

step no.2 - 2000 mg/kg body weight for 3 females - daths of all animals

Results and discussion

Effect levels

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 300 - < 2 000 mg/kg bw

Based on:

test mat.

Mortality:

step no.2 - 2000 mg/kg body weight for 3 females - daths of all animals

Clinical signs:

other:

Body weight:

lower than 10% body weight loss

Gross pathology:

group 1 and 3 wihout patological changes.
No.2 - piloerection, brightlight colour and market structure ofliver, light colour of kidny, stomach full of administrated test substance, dark cloudy urina. One animal was found dead with cyanotic skin, partly autolysed organs.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

According the study result the value of LD50of the test substance for female rats is in gange >300 - 2000mg/kg body weight.