[No public or meaningful name is available]

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

effects on growth of green algae

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20-28.4.2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2022

Report date:

2022

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

2006

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,3,3-tetrachloroprop-1-ene

EC Number:

675-808-5

Cas Number:

18611-43-3

Molecular formula:

C3H2Cl4

IUPAC Name:

1,1,3,3-tetrachloroprop-1-ene

Test material form:

liquid

Details on test material:

concentration 99,87%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

Algal growth medium (OECD Medium) containing 70 μL acetone in 700 mL solution was divided into 6 replicates, inoculated with algal cells (without test item) and examined in parallel to the test item concentrations and the untreated control.

Details on test solutions:

A stock solution (StS) was prepared by solving 2.5006 g test item in 5 mL acetone.
The test solutions were prepared by using 50 μL of each WS completed to 500 mL with OECD test
medium. The test solutions were then shaken for 5 minutes (300 rpm), and filtered through a 0.45 μm
filter.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Post exposure observation period:

none

Test conditions

Hardness:

fluorescent lamps were used with continuous lighting with light intensity of
7130-7380 lux.

Test temperature:

test temperature was
measured continuously with a min/max thermometer and remained between 22.5 and 23.8 °C. The
water temperature was determined at least daily in one vessel filled with water only. The water
temperature was measured to be between 22.9 and 23.5 °C.

pH:

7,37-8,88

Dissolved oxygen:

Not determined

Salinity:

none

Conductivity:

not determined

Nominal and measured concentrations:

The following nominal concentrations were tested: 0.15, 0.48, 1.53, 4.88, 15.63 and 50.0 mg/L.

Details on test conditions:

The pH varied by not more than 1.5 units of the controls. the test temperature was
measured continuously with a min/max thermometer and remained between 22.5 and 23.8 °C. The
water temperature was determined at least daily in one vessel filled with water only. The water
temperature was measured to be between 22.9 and 23.5 °C.

Reference substance (positive control):

yes

Remarks:

vehicle control group (10 μL acetone ad 100 mL OECD Medium), and untreated control group (OECD Test Medium without test item)

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 504 µg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

0,414 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the 72-h algal growth inhibition test on Raphidocelis subcapitata, the 72-h EC50
value based on growth rate was determined to be 0.504 mg/L and based on yield
was calculated to be 0.414 mg/L. The NOEC related to growth rates and yield was
determined to be 0.250 mg/L.

Executive summary:

In the 72-h algal growth inhibition test on Raphidocelis subcapitata, the 72-h EC50
value based on growth rate was determined to be 0.504 mg/L and based on yield
was calculated to be 0.414 mg/L. The NOEC related to growth rates and yield was
determined to be 0.250 mg/L.