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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

Currently viewing:

Administrative data

Endpoint:
skin sensitisation: in chemico
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2021

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 442C (In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA))
GLP compliance:
yes (incl. QA statement)
Type of study:
direct peptide reactivity assay (DPRA)

Test material

Constituent 1
Chemical structure
Reference substance name:
1-Methyl-1-propylpyrrolidinium Bis(fluorosulfonyl)imide
EC Number:
922-377-9
Cas Number:
852620-97-4
Molecular formula:
C8H18F2N2O4S2
IUPAC Name:
1-Methyl-1-propylpyrrolidinium Bis(fluorosulfonyl)imide
Test material form:
liquid

Results and discussion

In vitro / in chemico

Resultsopen allclose all
Key result
Group:
test chemical
Run / experiment:
run/experiment 1
Parameter:
lysine depletion
Value:
1.05 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
run/experiment 2
Parameter:
lysine depletion
Value:
5.55 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
run/experiment 3
Parameter:
lysine depletion
Value:
1.3 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
mean
Parameter:
mean lysine depletion
Value:
2.63 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
run/experiment 1
Parameter:
cysteine depletion
Value:
94.99 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Run / experiment:
run/experiment 2
Parameter:
cysteine depletion
Value:
95.48 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
run/experiment 3
Parameter:
cysteine depletion
Value:
93.58 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid
Key result
Group:
test chemical
Run / experiment:
mean
Parameter:
mean cystein depletion
Value:
94.68 %
At concentration:
100 mM
Negative controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
The test item show mean depletion of 2.63% for Lysine and 94.68% for Cysteine, i.e an overall average of 48.66% reflecting a high reactivity and therefore a positive prediction of DPRA.
The test methodDPRA is considered scientifically valid to be used as part of an integrated approaches to testing and assessment, to support the identification of the sensitization potential of test item for hazard classification and labeling purposes
Executive summary:

The test item show mean depletion of 2.63% for Lysine and 94.68% for Cysteine, i.e an overall average of 48.66% reflecting a high reactivity and therefore a positive prediction of DPRA. The test methodDPRA is considered scientifically valid to be used as part of an integrated approaches to testing and assessment, to support the identification of the sensitization potential of test item for hazard classification and labeling purposes