1-Methyl-1-propylpyrrolidinium Bis(fluorosulfonyl)imide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020-12-18 to 2021-02-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

See attached report

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia, Daphnia magna Strauss clone 5, between 6 and 24 hours old and produced from parthenogenically reproducing brood female population were used. Daphnia are issued from the breeding lab. The selection of the juvenile was daily realized by filtration.
The juvenile stemming at least from the second litter were used to start the test.

Culture conditions:
- Medium: Elendt M4
- Temperature: 20 °C ± 2
- Light/dark cycle: 16 h/8 h
- pH: Between 6 and 9
- Hardness (CaCO3): Between 140 and 250 mg.L-1
- Daily feeding: Pseudokirchneriella subcapitata and Chlorella vulgaris suspensions

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.88 to 20°C (mean: 19.94°C)

pH:

7.8

Dissolved oxygen:

8.1 to 8.4

Nominal and measured concentrations:

nominal: 0; 6.25; 12.5; 25.0; 50.0; 100 mg/L

Reference substance (positive control):

yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

95.04 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the EC50 after a 48-hour exposure period was estimated to be 95.04 mg test item.L-1.

Executive summary:

The aim of this study was to assess the effects of the test item N-Propyl-N-methylpyrrolidinium Bis(fluorosulfonyl)imide on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004). Daphnia magna is a freshwater crustacean that is recommended by the OECD Guideline 202.

The test item is a soluble product under the test conditions, so the test item solutions were prepared by by directly dissolving the test item in Elendt M4 medium.

The acute effects of N-Propyl-N-methylpyrrolidinium Bis(fluorosulfonyl)imide at the definitive nominal concentrations of 0; 6.25; 12.5; 25.0; 50.0 and 100 mg test item.L-1 to Daphnia magna were investigated under laboratory conditions.

The analytical samples were sampled at the beginning of the test and at the end of the test in the content of the vessels pooled and homogenised.

Since the deviation between the measured concentrations and the nominal concentrations was within the range of ± 20 %, analysis of the results was based on the nominal concentrations.

The EC50 and NOEC/LOEC values were based on the nominal concentrations.

 

	Result:
EC50(24h)	> 100 mg test item.L^-1
EC50(48h)	95.04 mg test item.L^-1

EC = effect concentration.

Description of key information

Under the test conditions, the EC50 after a 48-hour exposure period was estimated to be 95.04 mg test item.L-1.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

95.04 mg/L

Additional information

Experimental study performed according to the OECD 202 guideline and under GLP conditions.

Validity criteria were successful.

The study is considered acceptable for that endpoint.