1,1,3-trimethylindan-4-amine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04/2020-08/2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

8 mL of the synthetic medium and 100 mL of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 mL and aerated at 20 ± 2 °C.
The exposure medium with the reference substance was prepared by adding 8 mL of the synthetic medium, 100 mL of activated sludge and a defined amount of the stock solution to achieve the test concentrations, and was filled up with deionised water to 250 mL and aerated at 20 ± 2°C.
Control vessels (inoculated sample without test item) were prepared the same way.

Test organisms (species):

activated sludge, domestic

Details on inoculum:

aeration tank of a domestic waste water treatment plant (Odenthal)

Total exposure duration:

3 h

Test temperature:

20+/- 2°C

pH:

6-8

Nominal and measured concentrations:

10, 100, 1000 mg/L, 3 replicates
1000 mg/L with ATU, 2 replicates

Details on test conditions:

In the main test six controls without the test item were included in the test design, three at the start and the others at the end of the test series.
The test was performed with different test item concentrations with 3 replicates. Each batch of activated sludge was checked using 5 concentrations in the range of
2.5 - 40 mg/L of 3,5-Dichlorophenol as a reference compound.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

236.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

87.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

99.279% inhibition

Validity criteria fulfilled:

yes

Conclusions:

The respiration rate of each mixture was determined after aeration periods of 3 hours.

The EC50 is 236.3 mg/L.
The EC10 is 87.2 mg/L.
The NOEC is 32.0 mg/L.

The effect value relates to a nominal concentration, since no analytical monitoring was performed.

Executive summary:

The respiration rate of each mixture was determined after aeration periods of 3 hours.

 

The EC₅₀is 236.3 mg/L.

The EC₁₀is 87.2 mg/L.
The NOEC is 32.0 mg/L.

 

The effect value relates to a nominal concentration, since no analytical monitoring was performed.

Description of key information

The respiration rate of each mixture was determined after aeration periods of 3 hours.

 

The EC₅₀is 236.3 mg/L.

The EC₁₀is 87.2 mg/L.
The NOEC is 32.0 mg/L.

 

The effect value relates to a nominal concentration, since no analytical monitoring was performed.

Key value for chemical safety assessment

EC50 for microorganisms:

236.3 mg/L

EC10 or NOEC for microorganisms:

32 mg/L