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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
Test procedure in accordance with national standard methods, study not sufficiently documented.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DEV, DIN 38412, L3
Version / remarks:
national standard method
Principles of method if other than guideline:
Determination of the dehydrogenase acitivaty (DHA) using 2,3,5-triphenyltetrazoliumchloride (TTC) in compliance with the german procedure L3.
The test has been performed according to German Procedure (DEV) for Examination of Water, Effluent and Sludge 7, 1975 Version and Wellens and Zahn: Investigations into the Toxicity Determination of Effluents (TTC Method). Chemiker-Zeitung, 1971, No. 10, p.472.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
EC Number:
211-322-8
EC Name:
1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
Cas Number:
638-16-4
Molecular formula:
C3H3N3S3
IUPAC Name:
1,3,5-triazine-2,4,6(1H,3H,5H)-trithione

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Test substance concentration have been prepared by filling up to 1000 mL test volume with chlorine-free tap water. The samples have been pretrated by neutralizatoin to a pH value of 7.5 +/- 0.2.

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

pH:
Neutralization to pH 7.5 +/- 0.2 (20 °C)
Nominal and measured concentrations:
Nominal: 1, 10, 50, 100, 200, 400, 500, 600, 800 and 1000 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dehydrogenase activity
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dehydrogenase activity
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
ca. 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dehydrogenase activity

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified