N,N'-bis(3-aminopropyl)propane-1,3-diamine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific standards, acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

observation period: 8 days

Principles of method if other than guideline:

The skin irritation test was conducted according to a BASF-internal standard protocol. White Vienna rabbits were treated with the substance for 1 min, 5 min, 15 min, or 20 h, respectively, under occlusive conditions.
There were two experiments performed.
During the first experiment two rabbits were exposed to the test substance for 1 min, 5 min, or 15 min on their backs.
During the second experiment two rabbits were exposed to the test substance for 20 h on their backs and their ears.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

EC Number:

225-007-8

EC Name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

Cas Number:

4605-14-5

Molecular formula:

C9H24N4

IUPAC Name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.45, 2,77, 3.7 and 2.95 kg

Test system

Type of coverage:

not specified

Preparation of test site:

not specified

Vehicle:

unchanged (no vehicle)

Controls:

no

Duration of treatment / exposure:

The first experiment :1 min, 5 min, 15 min
The second experiment: 20 h

Observation period:

8 d

Number of animals:

4

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: yes (exposure 1, 5, 15 min) first with undiluted Lutrol, then with an aqueous Lutrol solution (50 %); not specified in the second experiment (exposure 20 h)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24 h

Score:

>= 2

Reversibility:

not reversible

Remarks on result:

other: full thickness necrosis observed after 15 min exposure.

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24 h

Score:

>= 2

Reversibility:

not reversible

Remarks on result:

other:

Remarks:

full thickness necrosis obsered after 15 min of exposure.

Applicant's summary and conclusion

Interpretation of results:

Category 1B (corrosive) based on GHS criteria

Conclusions:

The application of the test substance to rabbit skin caused full thickness necrosis. Exposure duration: 15 min. Reading: 24 h post application.