N,N'-bis(3-aminopropyl)propane-1,3-diamine

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 406 (Skin Sensitisation)

GLP compliance:

no

Type of study:

skin painting test

Justification for non-LLNA method:

The present study was performed in 1970, before the LLNA and/or any in vitro skin sens-tests were available

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

EC Number:

225-007-8

EC Name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

Cas Number:

4605-14-5

Molecular formula:

C9H24N4

IUPAC Name:

N,N'-bis(3-aminopropyl)propane-1,3-diamine

In vivo test system

Test animals

Species:

guinea pig

Strain:

not specified

Sex:

female

Details on test animals and environmental conditions:

no data

Study design: in vivo (non-LLNA)

No. of animals per dose:

10

Details on study design:

The area of the front right and left flank of 10 white guinea pigs was depilated in an area of approx. 25 cm2. After 4 hours the left flank was degreased with ether and pinned three times in a row in cross shape with a 1% and 5% acetonic solutions of the products soaked cotton wool. This was happening on a daily basis. After a break of 10 days, during the skin changes caused by this treatment reversed, both flanks were again shaved, 4 hours later, the right, previously untreated side after degreasing with ether was coated once with a 1%, primarily irritant acetonic solution in cross form and the skin reaction was registered after 12 hours.

Challenge controls:

yes, concurrent treatment of 3 previously untreated guinea pigs

Positive control substance(s):

no

Results and discussion

In vivo (non-LLNA)

Results

Key result

Reading:

1st reading

Hours after challenge:

12

Group:

test chemical

Dose level:

1%

No. with + reactions:

0

Total no. in group:

10

Remarks on result:

no indication of skin sensitisation

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Through the repeated application of 1% and 5% acetonic solution of tripropylenetetramine bloody crusts were formed in the application area. The challange with a 1% acetonic solution of tripropylenetetramine did not induce skin sensitisation.