2,4-difluoroaniline

Toxicity to soil microorganisms

Administrative data

Endpoint:

toxicity to soil microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)

Deviations:

yes

Remarks:

The soil water content maintained at the starting value within a range of ± 5 % for all sampling dates and rates, except day 28, when the deviation was 8% for the control and 6% for test rate 2.

Principles of method if other than guideline:

The soil water content maintained at the starting value within a range of ± 5 % for all sampling dates and rates, except day 28, when the deviation was 8% for the control and 6% for test rate 2. The deviation during incubation time was justifiable and occurred only in control and test rate 2 at day 28. Most time the water content was in the desired range.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-difluoroaniline

EC Number:

206-687-5

EC Name:

2,4-difluoroaniline

Cas Number:

367-25-9

Molecular formula:

C6H5F2N

IUPAC Name:

2,4-difluoroaniline

Sampling and analysis

Analytical monitoring:

yes

Test substrate

Vehicle:

yes

Remarks:

acetone

Test organisms

Test organisms (inoculum):

soil

Study design

Total exposure duration:

28 d

Test conditions

Nominal and measured concentrations:

Negative control, Sovlent control, 0.05, 0.25 mg/kg soil dw (nominal)

Results and discussion

Effect concentrations

Remarks on result:

other: No impact of the test item on nitrogen transformation (nitrate content, mineral nitrogen content and nitrate formation rate) of soil microorganisms.

Details on results:

The test item had no impact on nitrogen transformation (nitrate content, mineral nitrogen content and nitrate formation rate) of soil microorganisms when applied at 0.05 mg and 0.25 mg test item/kg soil dw.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item had no impact on nitrogen transformation (nitrate content, mineral nitrogen content and nitrate formation rate) of soil microorganisms when applied at 0.05 mg and 0.25 mg test item/kg soil dw.