Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-057-9 | CAS number: 91-29-2
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data from secondary source
Data source
Referenceopen allclose all
- Reference Type:
- other: Authoritive database
- Title:
- Acute dermal toxicity for CAS NO: 91-29-2
- Author:
- IFA GESTIS
- Year:
- 2 017
- Bibliographic source:
- GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn).
- Reference Type:
- secondary source
- Title:
- Toxicological evaluations -4-Nitro-4’-aminodiphenylamine-2-sulfonic acid
- Author:
- BG Chemie
- Year:
- 2 000
- Bibliographic source:
- BG Chemie InfoCenter TOXIKOLOGISCHE BEWERTUNGEN; 06/2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Principles of method if other than guideline:
- An acute dermal toxicity study of 4-nitro-4’ aminodiphenylamine-2-sulfonic acid was carried out according to OECD guideline No. 402 in 5 male and 5 female rats to determine its LD50.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- yes
Test material
- Reference substance name:
- 2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
- EC Number:
- 202-057-9
- EC Name:
- 2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
- Cas Number:
- 91-29-2
- Molecular formula:
- C12H11N3O5S
- IUPAC Name:
- 2-(3-(4-amino-9,10-dihydro-3-sulpho-9,10-dioxoanthracen-4-yl)aminobenzenesulphonyl)vinyl) disodium sulphate
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (91-29-2)
- Molecular formula: C12H11N3O5S
- Molecular weight : 309.301 g/mol
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid
- Molecular formula: C12H11N3O5S
- Molecular weight: 309.301 g/mol
- Substance type: Organic
- Physical state: Solid
Test animals
- Species:
- rabbit
- Strain:
- other: Tif:Raif
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- - Weight at study initiation: 184-224g
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- other: Distilled water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: the shaved dorsal skin - Duration of exposure:
- not specified
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- Total :10
5 male
5 female - Control animals:
- not specified
- Details on study design:
- - Details on study design
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: No data available
- Necropsy of survivors performed: yes
- Other examinations performed: No data available - Statistics:
- No data available
Results and discussion
- Preliminary study:
- No data available
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was not observed.
- Mortality:
- All animals survived
- Clinical signs:
- The symptoms observed included dyspnea, ruffled fur, curved and ventral body position as well as transient body position
- Body weight:
- No data available
- Gross pathology:
- Autopsy revealed no unusual findings.
- Other findings:
- No data available
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified
- Conclusions:
- The acute dermal toxicity LD 50 was considered to be >2000 mg/kg to rat when treated with 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (91-29-2).
- Executive summary:
An acute dermal toxicity study of 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (91-29-2).was carried out according to OECD guideline No. 402.
Each of 5 male and 5 female rats (Tif:RAIf, SPF; initial weight 184 to 224 g) received a single semi-occlusive application to the shaved dorsal skin of 2000 mg of 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (70.3%) per kg body weight in a 50% formulation with distilled water. The observation period was 14 days.
All animals survived. The symptoms observed included dyspnoea, ruffled fur, curved and ventral body position as well as transient sedation and diarrhoea. There were no local reactions. Autopsy revealed no unusual findings.
Therefore the acute dermal toxicity LD 50 value for4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) in Wistar rat was considered to be > 2000 mg/kg. When rats were treated with 4-nitro-4’-aminodiphenylamine-2-sulfonic acid by dermal application.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
