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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from handbook or collection of data

Data source

Reference
Reference Type:
other: Authoritive database
Title:
Eye irritation for CAS NO: 91-29-2
Author:
IFA GESTIS
Year:
2017
Bibliographic source:
GESTIS SUBSTANCE Database (information system in hazardous substance of the Berufsgenossenscheftn).

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
Ocular irritation study of 4-nitro-4’-aminodiphenylamine-2-sulfonic acid was carried out in 6 rabbits in accordance with OECD guideline No. 405
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
EC Number:
202-057-9
EC Name:
2-(4-aminoanilino)-5-nitrobenzenesulphonic acid
Cas Number:
91-29-2
Molecular formula:
C12H11N3O5S
IUPAC Name:
2-(3-(4-amino-9,10-dihydro-3-sulpho-9,10-dioxoanthracen-4-yl)aminobenzenesulphonyl)vinyl) disodium sulphate
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (91-29-2)
- Molecular formula: C12H11N3O5S
- Molecular weight : 309.301 g/mol
- Substance type: Organic
- Physical state: Solid

Specific details on test material used for the study:
- Name of test material (as cited in study report): 4-nitro-4’-aminodiphenylamine-2-sulfonic acid
- Molecular formula: C12H11N3O5S
- Molecular weight: 309.301 g/mol
- Substance type: Organic
- Physical state: Solid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
water
Controls:
not specified
Amount / concentration applied:
100 µl

Duration of treatment / exposure:
3 days
Observation period (in vivo):
3 days
Duration of post- treatment incubation (in vitro):
No data available
Number of animals or in vitro replicates:
6
Details on study design:
TEST SITE
- Area of exposure: the conjunctival sac of eye
- % coverage: No data
- Type of wrap if used: No data

REMOVAL OF TEST SUBSTANCE
- Washing (if done): No data
- Time after start of exposure: No data
SCORING SYSTEM: No data

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 3 days
Score:
0
Reversibility:
fully reversible
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
Slight reddening in only one of 6 animals was observed but this was reversible after 3 days.

Applicant's summary and conclusion

Interpretation of results:
other: not irritating
Conclusions:
4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) was considered to be not irritating to eye of rabbits.
Executive summary:

Eye irritation study was conducted in 6 rabbits for 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No: 91-29-2) to assess its eye irritation efficacy in accordance with OECD guideline 405. About 100 μl of 4-nitro-4’-aminodiphenylamine-2-sulfonic acid was placed into the conjunctival sac of each rabbits and the results were recorded for 3 days. Slight reddening in only one of 6 animals was observed but this was reversible after 3 days.

Since the observed eye effects were not persisted after 3 days ,the test chemical 4-nitro-4’-aminodiphenylamine-2-sulfonic acid (CAS No:91-29-2) was considered to be not irritating to rabbits’ eye.