Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-289-5 | CAS number: 56-81-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa-term.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted prior to GLP and test guidelines, but sufficient data is available for interpretation of results
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The biological degradation of 123 organic compounds was studied.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- Activated sludge obtained from a sewage plant was used. Starch or glucose and peptone were the only chemicals added during the 20 day adaptation phase.
- Duration of test (contact time):
- ca. 24 h
- Details on study design:
- The tested substance is dissolved in a beaker in a biological medium in a concentration corresponding to 200mg/L COD. The tested substance is a sole source of organic carbon for the microbes of the inoculum. To the biological medium such amount of thickened adapted activated sludge is added to make dry matter of the inocuium 100 mg/L. The beaker is placed in a dark room with a roughly constant temperature of 20+/- 3 C on an electromagnetic stirrer. The initial value of COD or organic carbon of the liquid phase are determined. Samples. filtered or centrifuged before analysis, are taken at suitable intervals. The decrease of the tested substance in the liquid phase is evaluated by determining COD or organic carbon. The rest, Its are compared with those of a blank test and standard compound decomposition. With the degree of degradation also the average specific rate of degradation is determined, expressed in terms of mg COD (or organic carbon) removed by a gamme of dry matter of the activated sludge per hour.
- Reference substance:
- diethylene glycol
- Reference substance:
- aniline
- Preliminary study:
- Not applicable.
- Test performance:
- 98.7% of the glycerol was removed within approximately 24 hours based on COD. The rate of biodegradation was 85.0 mg COD g-1 h-1.
- Parameter:
- % degradation (DOC removal)
- Value:
- 98.7
- Sampling time:
- 24 h
- Details on results:
- No additional information available.
- Results with reference substance:
- 95% of the diethylene glycol was removed based on COD measurement (time interval not specified but less than 20 days)
94.5% of the aniline was removed based on COD measurement (time interval not specified but less than 20 days). - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In this study, glycerol was rapidly biodegraded. 98.7% of the glycerol was removed within approximately 24 hours based on COD. The rate of biodegradation was 85.0 mg COD/g/h.
- Executive summary:
In this study, glycerol was rapidly biodegraded. 98.7% of the glycerol was removed within approximately 24 hours based on COD. The rate of biodegradation was 85.0 mg COD/g/h.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was not conducted according to guideline/s and GLP but the report contains sufficient data for interpretation of study results
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The biodegradation potential of glycerin was examined using activated sludge from an industrial source.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
ethylene glycol was used as positive control - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Details on inoculum:
- - Inoculum (source/concentration): activated sludge
(industrial)
- pretreatment: washed with tap water
METHOD OF PREPARATION OF TEST SOLUTION: not indicated - Duration of test (contact time):
- 24 h
- Initial conc.:
- 226 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- INITIAL TEST SUBSTANCE CONCENTRATION: 88 mg C/L (=226 mg TS/L)
TEST SYSTEM
- Test apparatus: fill and draw type unit; one with two aeration cylinders, each with a volume of 7 L, the other with two aeration cylinders, each with a volume of 30 L (no more information available in this report, Nanbu(1971) is given as a reference)
- Number of replicates: not indicated
- Aeration: yes (5 L/min)
- Measuring equipment:
TOC: TOC analyser
COD-Mn: water sample was oxidised with KMnO4-H2SO4 at 100 C for 30 minutes (catalyst: Ag2SO4)
DURATION OF THE TEST: 24 hours
SAMPLING: 0, 2, 4, 24 hours
ANALYTICAL PARAMETER: TOC and COD-Mn
ThOD: 1.21 mg/g = 275 mg/L (COD = 238 mg/L)
TEST CONDITIONS
- Test temperature: 25 C
CONTROLS: not included
REFERENCE SUBSTANCE: ethylene glycol was also included in this test and can be used as a positive control - Reference substance:
- ethylene glycol
- Preliminary study:
- No additional information available.
- Parameter:
- % degradation (TOC removal)
- Value:
- 60
- Sampling time:
- 2 h
- Parameter:
- % degradation (TOC removal)
- Value:
- 86
- Sampling time:
- 4 h
- Parameter:
- % degradation (TOC removal)
- Value:
- 94
- Sampling time:
- 24 h
- Details on results:
- Probably no blank was included in the test, but it was stated, that the contribution of TOC and COD-Mn from the activated sludge was negligible.
Removal test substance as %COD, %TOC respectively:
0 hour: 0, 0
2 hour: 50, 60
4 hour: 92, 86
24 hour: 97, 94 - Results with reference substance:
- REFERENCE SUBSTANCE
Removal test substance as %COD, %TOC respectively:
0 hour: 0, 0
2 hour: 14, 18
4 hour: 32, 38
24 hour: 92, 93 - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test material was essentially biodegraded within 24 hours.
- Executive summary:
The biodegradation of glycerin was examined using industrial activated sludge. Glycerin was nearly completely degraded within 24 hours. No indication is given in the report whether the microorganisms were pre-adapted. However, given that glycerin is considered a primoridial biomolecule from which many biomolecules are derived, pre-adaptation is probably minimal.
Referenceopen allclose all
No additional information available.
No additional information available.
Description of key information
The biodegradation of glycerin was examined using industrial activated sludge. Glycerin was nearly completely degraded within 24 hours. No indication is given in the report whether the microorganisms were pre-adapted. However, given that glycerin is considered a primoridial biomolecule from which many biomolecules are derived, pre-adaptation is probably minimal.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The biodegradation of glycerin was examined using industrial activated sludge. Glycerin was nearly completely degraded within 24 hours. No indication is given in the report whether the microorganisms were pre-adapted. However, given that glycerin is considered a primoridial biomolecule from which many biomolecules are derived, pre-adaptation is probably minimal.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)