Registration Dossier
Registration Dossier
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EC number: 217-589-7 | CAS number: 1897-52-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 = 113 mg/l, Brachydanio rerio; OECD 203, EPA 797.1440; Berends AG, Thus JLG, 1992
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 113 mg/L
Additional information
The acute toxicity of the test material to fish was investigated in Brachdanio rerio (zebra fish) in accordance with the standard guidelines OECD 203 and EPA 797.1440. The test was conducted at nominal test concentrations of 0, 29, 51, 93, 170 and 300 mg/l. At each test concentration ten fish were exposed for 96 hours in 4 litres of test solution. The test solutions were renewed daily. The concentrations of the test solutions were measured with HPLC, both before and after renewal. The results showed generally a good agreement between nominal and measured concentrations. the biological results are based on mean measured concentrations.
At a concentration of 84.4 mg/l no mortality was found after 96 hours. At 152 mg/l 100% mortality was observed. The LC50 was estimated by calculating the geometric mean of 84.4 and 152 mg/l, which resulted in an LC50 of 113 mg/l. The interval between 84.4 and 152 mg/l can be considered a 95% confidence interval for the LC50. Considering sublethal effects on behaviour and appearance of the fish a concentration of 44.8 mg/l is a No Effect Concentration.
The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. the study was assigned a reliability score of 1 and considered suitable for assessment as an acurate reflection of the test substance.
The available data is considered to be complete and the result determined, 96 h LC50 of 113 mg/l, was taken forward for risk assessment.
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