N-tert-butylacrylamide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication

Data source

Reference

Reference 1

Reference Type:

publication

Title:

To determine acute oral toxicity of test chemical in mice

Author:

Kazuo Hashimoto et. al.

Year:

1981

Bibliographic source:

Archives of Toxicology

Reference 2

Reference Type:

review article or handbook

Title:

ACUTE ORAL TOXICITY STUDY IN MICE

Author:

Richard J. Lewis

Year:

2012

Bibliographic source:

Sax's Dangerous Properties of Industrial Materials, 12th Edition, 5 Volume Set,2012

Reference 3

Reference Type:

other: Authoritive database

Title:

Acute oral toxicity study of test chemical in mice

Author:

U.S. National Library of Medicine

Year:

2018

Bibliographic source:

Chemidplus Database,U.S. National Library of Medicine,2018

Reference 4

Reference Type:

secondary source

Title:

acute oral toxicity study of test chemical in mice

Author:

RTECS

Year:

2018

Bibliographic source:

RTECS (registry of toxic effect of chemical substance database), 2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

The acute toxicity of the test chemical by oral administration in mice was determined according to Weil (1952), using four animals per dosage level and four different doses.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-tert-butylacrylamide

EC Number:

203-505-6

EC Name:

N-tert-butylacrylamide

Cas Number:

107-58-4

Molecular formula:

C7H13NO

IUPAC Name:

N-tert-butylacrylamide

Details on test material:

- Name of test material (IUPAC name): 2-Propenamide, N-(1,1-dimethylethyl)-
- Common name: N-tert-butylacrylamide
- Molecular formula: C7H13NO
- Molecular weight: 127.1857g/mol
- Smiles notation: CC(C)(C)NC(=O)C=C
- InChl: 1S/C7H13NO/c1-5-6(9)8-7(2,3)4/h5H,1H2,2-4H3,(H,8,9)
- Substance type: Organic

Test animals

Species:

mouse

Strain:

other: ddY

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 5-6 weeks
- Weight at study initiation: 29 2.2 g body weight
- Housing: Animals were randomly placed in plastic cages (5-7 per cage) containing wooden flakes.
- Diet (e.g. ad libitum): laboratory chow, ad libitum
- Water (e.g. ad libitum): water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

DMSO

Details on oral exposure:

no data

Doses:

5.6 - 9.7 mmol/kg (712.23 - 1233.7 mg/kg bw)

No. of animals per sex per dose:

4 animals per dose

Control animals:

not specified

Details on study design:

no data

Statistics:

The acute toxicity of the test compounds by oral administration in mice was determined according to Weil (1952).

Results and discussion

Preliminary study:

no data

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

941.174 mg/kg bw

Based on:

test mat.

95% CL:

712.23 - 1 233.7

Remarks on result:

other: 50% mortality was observed

Mortality:

50% Mortality was observed in treated mice at 941.174 mg/kg bw.

Clinical signs:

other: no data

Gross pathology:

no data

Other findings:

no data

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The lethal concentration (LD50) value for acute oral toxicity test was considered to be 914.174 mg/kg bw, with 95% confidence limit of 712.23 - 1233.7 mg/kg, when male ddy strain mice were treated with the given test chemical orally.

Executive summary:

Acute oral toxicity study was performed by using the given test chemical in Male mice of ddY strain at the dose concentration of 5.6 - 9.7 mmol/kg (712.23 - 1233.7 mg/kg bw). The given test chemical was dissolved in DMSO and administered via oral route. Animals were 5-6 weeks of age and 29 ± 2.2 g body weight at the beginning of the experiment and were randomly placed in plastic cages (5-7 per cage) containing wooden flakes. They were fed laboratory chow and water ad libitum. The acute toxicity of the test compounds by oral administration in mice was determined according to Weil (1952). 50% Mortality was observed in treated mice at 941.174 mg/kg bw. Therefore, the lethal concentration (LD50) value for acute oral toxicity test was considered to be 914.174 mg/kg bw, with 95% confidence limit of 712.23 - 1233.7 mg/kg, when male ddy strain mice were treated with the given test chemical orally.