2-aminoethyldiethylamine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable study report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

not specified

Principles of method if other than guideline:

According to BASF-internal standard, see details in the section "Any other information on materials and methods incl. tables".

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-aminoethyldiethylamine

EC Number:

202-844-7

EC Name:

2-aminoethyldiethylamine

Cas Number:

100-36-7

Molecular formula:

C6H16N2

IUPAC Name:

N1,N1-Diethyl-1,2-ethanediamine

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

Mean body weights of the rabbits: 2.85 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin of the same animal served as control.

Amount / concentration applied:

ca. 1 mL

Duration of treatment / exposure:

3 minutes

Observation period:

8 days

Number of animals:

2

Details on study design:

Application area: 2.5 x 2.5 cm

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 - 48 h

Score:

4

Max. score:

4

Reversibility:

not reversible

Remarks on result:

other: parchment-like necrosis (comprehensive)

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 - 48 h

Score:

2

Max. score:

2

Reversibility:

not reversible

Remarks on result:

other: comprehensive

Results 3

Irritation parameter:

erythema score

Basis:

animal: #1, #2

Time point:

other: 3 min

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: necrosis observed, overarching skin reaction

Results 4

Irritation parameter:

edema score

Basis:

animal: #1, #2

Time point:

other: 3 min

Score:

2

Max. score:

2

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: overarching skin reaction

Other effects:

Pathology: The skin effects of the sacrificed animals were macroscopically confirmed.

Applicant's summary and conclusion

Interpretation of results:

highly corrosive

Remarks:

Migrated information Criteria used for interpretation of results: EU

Executive summary:

In a skin irritation/corrosion study (BASF, 1980), 2 Vienna White rabbits were treated with undiluted test substance for 3 min using occlusive conditions. An application site of 2.5 cm² was covered with ca. 1 mL of the liquid test substance. The animals were observed for 8 days and skin changes were recorded daily. For a final evaluation, the findings from 24 and 48 hours from the raw data were taken into account on a mean basis (erythema score, edema score). The application for 3 min evoked after 3 min strong skin reactions and caused after 24 hours leathery-like necrosis in both animals. Parchment-like necrosis was observed after 8 days. This study is classified as acceptable. It generally satisfies the guideline requirements for OECD 404.