Hexanoic acid, 3,5,5-trimethyl-, 2,2,6,6-tetramethyl-1-[2-[(3,5,5-trimethyl-1-oxohexyl)oxy]ethyl]-4-piperidinyl ester

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-02-27 to 2018-09-20

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-{2,2,6,6-tetramethyl-4-[(3,5,5-trimethylhexanoyl)oxy]piperidin-1-yl}ethyl 3,5,5-trimethylhexanoate

EC Number:

941-267-1

Cas Number:

1445870-18-7

Molecular formula:

C29H55NO4

IUPAC Name:

2-{2,2,6,6-tetramethyl-4-[(3,5,5-trimethylhexanoyl)oxy]piperidin-1-yl}ethyl 3,5,5-trimethylhexanoate

Test material form:

liquid

Details on test material:

- Appearance: Yellowish to brownish liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The test substance concentrations of the blank control and the treatment group were sampled and analyzed when freshly prepared and prior to renewal during the firstweek of the test. 2OmL test solution was taken respectively from the freshly prepared test solutions and the solutions prior to renewal at each group and filtrated with O.22pm cellulose nitrate membrane filters. In Week 2 and 3, the sampling was operationed twice a week, once after renewal, and the next before renewal.
The samples were stored in refrigerator (2-8'C) after pretreatment (see 2.2.7) if thesamples were not analyzed directly in 24h.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Range-finding test
According to the information provided by the sponsor, test substance is insoluble in water ( <0.4µg/L), 2L solution of the test substance, with a nominal concentration of 10 mg/L, were prepared with Elendt M4 medium and stirred (via magnetic stirrer) in the dark for 24h with the vortex depth of 1/3 for the solution, and were filtrated with 0.45 µm nitrocellulose membrane to make a 100% saturated solution of the test substance in the test water (the first 100mL of the filItrate were discarded), which was used as the stock solution and the test solution of the highest treatment group, The test medium of the control group was treated in exactly the same way as the highest treatment group, including stirring and filtration. The test solutions of the other treatment groups were prepared by diluting the 100% saturated solution with Elendt M4 medium.
The pH of the stock solution measured during the test was 8.061 - 8.165, not exceeding 6 - 9, and needed not to be adjusted.

Limit test
2L solution of the test substance, with a nominal concentration of 10 mg/L, was prepared with Elendt M4 medium and stirred (via magnetic stirrer) in the dark for 24 hours with the vortex depth of 1/3 for the solution, and were filtrated with 0.45 µm nitrocellulose membrane to make a 100% saturated solution of the test substance in the test water (the first 100mL of the filtrate were discarded), which was used as the test solution of the teratment group. The test medium of the control group was treated in exactly the same way as the treatment group, including stirring and filtration.
The pH of the treatment group measured during the test was 8.208 - 8.339, not exceeding 6 - 9, and needed not to be adjusted.

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

NOEC

Remarks on result:

not determinable