Reaction mass of Benzeneethanol, 2-chloro-α-(1-chlorocyclopropyl)-α-(chloromethyl)- and Oxirane, 2-(1-chlorcyclopropyl)-2-[(2-chlorphenyl)methyl]-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

GC-Chromatography

Details on sampling:

Sampling schedule of chemical analysis:
- Control: at 0 and 48 hours
- Test concentrations: at 0 and 48 hours

Vehicle:

no

Details on test solutions:

To give the desired series of test concentrations direct weighings were prepared for the test concentrations 0.5, 1, 2 and 4 mg/L. The test substance was added to dilution water and treated with an ultra turrax for 60 seconds at 8000 rpm and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration using folded filters of pore size 7-12 μm. The test concentration 0.25 mg/L was prepared by a dilution of the test concentration 0.5 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Name: Daphnia magna STRAUS, parthenogenetic females
- Source: Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semiquantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

14.4 °dH (257.04 mg/L CaCO3)

Test temperature:

20.0 - 20.2 °C

pH:

7.4 - 7.6

Dissolved oxygen:

7.3 - 8.1 mg/L (concerning the highest test concentration)

Salinity:

n.a.

Conductivity:

No data

Nominal and measured concentrations:

Test concentration/s (nominal): 0.25, 0.5, 1, 2 and 4 mg/L
Measured concentrations ranged from 42 - 86% of nominal values at 0 hours, and from 41 - 84 % of nominal values at 48 hours

Details on test conditions:

Exposure conditions:
- Test vessels: Karlsruher Flaschen holding 10 neonates in 250-300 mL of test medium 3
- Experimental design: 5 test concentrations plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period; closed bottle conditions
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light: 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 18 - 22 °C)
- Aeration: none
- Method of administration: direct weighing and stock solution
- Medium renewal: none
- Criteria of effects: The criterion of adverse effects used in this study was the substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.39 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

0.215 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

0.71 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 42 - 86% of nominal values at 0 hours, and from 41 - 84 % of nominal values at 48 hours, respectively.

Validity criteria fulfilled:

yes

Remarks:

immobilisation in controls <= 10% by the end of the test; dissolved oxygen concentration >3 mg/L throughout the exposure period

Conclusions:

After 48 hours of exposure of the daphnids to the test substance an EC0 of 0.215 mg/L and an LC100 of 0.71 mg/L were determined. The geometric mean of the two values corresponding to the EC50 value is 0.39 mg/L. The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 42 - 86% of nominal values at 0 hours, and from 41 - 84 % of nominal values at 48 hours.

Executive summary:

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. Daphnia were exposed to a range of concentrations, nominally 0.25, 0.5, 1, 2 and 4 mg/L of test item dissolved in water. 2 vessels per concentration with 10 daphnids each were used. During the test a temperature range of 18 - 22°C is to be maintained in the test vessels, with a maximum temperature fluctuation of+/- 1 °C in each individual test. pH and oxygen values are measured at the end of the test. After 48 hours of exposure an EC0 of 0.215 mg/L and an EC100 of 0.71 mg/L were determined. The geometric mean of the two values corresponding to the EC50 value is 0.39 mg/L.

The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 42 - 86% of nominal values at 0 hours, and from 41 - 84 % of nominal values at 48 hours.

Description of key information

A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions in a closed bottle system. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'. Daphnia were exposed to a range of concentrations, nominally 0.25, 0.5, 1, 2 and 4 mg/L of test item dissolved in water. 2 vessels per concentration with 10 daphnids each were used. During the test a temperature range of 18 - 22°C is to be maintained in the test vessels, with a maximum temperature fluctuation of+/- 1 °C in each individual test. pH and oxygen values are measured at the end of the test. After48 hours of exposure an EC0 of 0.215 mg/L and an EC100 of 0.71 mg/L were determined. The geometric mean of the two values corresponding to the EC 50 value is 0.39 mg/L.

The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 42 - 86% of nominal values at 0 hours, and from 41 - 84 % of nominal values at 48 hours.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.39 mg/L