Reaction mass of Benzeneethanol, 2-chloro-α-(1-chlorocyclopropyl)-α-(chloromethyl)- and Oxirane, 2-(1-chlorcyclopropyl)-2-[(2-chlorphenyl)methyl]-

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Type: mixed population of aquatic microorganisms
- Source of sewage effluent: secondary effluent of a domestic sewage treatment plant (Odenthal)
- Date of collection: 2002-01-18
- Preconditioning: seperation of coarse particles by filtration, aeration of mineral medium plus inoculum (filtrate) for 7 days
- Effluent concentration in reaction mixture: 5 mL/L

Duration of test (contact time):

28 d

Initial conc.:

3.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Pretreatment of the test substance:
- The test substance was weighed out on aluminium foil. This substance inclusive the aluminium foil was added to the test vessels.
- Initial concentration of the test substance 3.8 mg test substance/L
- Initial concentration of the reference compound : 2.9 reference compound/L

Exposure conditions:
- Incubation time: 28 days
- Incubation temperature: 20 ± 1 °C
- inoculum blank and toxicity control were run in parallel

Reference substance:

benzoic acid, sodium salt

Remarks:

Purity 99 %

Parameter:

% degradation (O2 consumption)

Value:

3

Sampling time:

7 d

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

14 d

Parameter:

% degradation (O2 consumption)

Value:

3

Sampling time:

21 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

28 d

Results with reference substance:

The reference compound showed 84 % degradation after 14 days.

Toxicity control:

3.8 mg/L test substance and 2.9 mg/L reference substance did not show toxic effects to bacteria. A degradation of 34 % ThOD was determined after 14 d.

Validity criteria fulfilled:

yes

Remarks:

for details see 'overall remarks'

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The substance showed 2 % degradation after 28 days. Therefore the substance has to be classified as "Not Readily Biodegradable".

Executive summary:

The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-E: Closed Bottle Test. This test method is in all essential parts identical with OECD Guideline 301 D. The test substance in a mineral medium was inoculated with secondary effluent and incubated for 28 d under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. A blank control, a toxicity control and a reference test were run in parallel. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum, is expressed as a percentage of theoretical oxygen demand (ThOD) or chemical oxygen demand (COD). The substance showed 2 % degradation after 28 days. Therefore the substance has to be classified as "Not Readily Biodegradable".

The reference compound showed 84 % degradation after 14 days.

Description of key information

The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-E: Closed Bottle Test. This test method is in all essential parts identical with OECD Guideline 301 D. The test substance in a mineral medium was inoculated with secondary effluent and incubated for 28 d under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. A blank control, a toxicity control and a reference test were run in parallel. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum, is expressed as a percentage of theoretical oxygen demand (ThOD). The substance showed 2 % degradation after 28 days. Therefore the substance has to be classified as "Not Readily Biodegradable".

The reference compound showed 84 % degradation after 14 days.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater