1,3-Benzenedicarboxamide, 5-amino-N1,N3-bis(2,3-dihydroxypropyl)-, hydrochloride (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

421-690-3

EC Name:

-

Cas Number:

203515-86-0

Molecular formula:

C14H21N3O6 . ClH

IUPAC Name:

5-amino-N1,N3-bis(2,3-dihydroxypropyl)benzene-1,3-dicarboxamide hydrochloride

Constituent 2

Reference substance name:

3,5-bis[(2,3-dihydroxypropyl)carbamoyl]aniliniumchloride

IUPAC Name:

3,5-bis[(2,3-dihydroxypropyl)carbamoyl]aniliniumchloride

Details on test material:

- Name of test material (as cited in study report): ABA-HCl
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type:
- Physical state: Powder
- Analytical purity: ≥98.5
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.: S-31308
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions:
- Storage condition of test material:
- Other:

Test organisms

Test organisms (species):

Daphnia magna

Study design

Water media type:

freshwater

Total exposure duration:

24 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

0.3 g/L

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

0.3 g/L

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LC0

Effect conc.:

0.3 g/L

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

0.5 g/L

Effect concentrations 5

Duration:

24 h

Dose descriptor:

LC100

Effect conc.:

0.7 g/L

Applicant's summary and conclusion

Conclusions:

The range of the EC50 (24h) was 370 -670 mg/l, the EC100(24h) 700 mg/l and the NOEC (24h) 300mg/l. Despite missing measurements of the test substance the test was accepted due to the extensive measurements of the test concentrations in the fish test (showing less than 5% loss over 96 h) and 24 h were accepted due to the high EC50 values. Two tests were performed. One with adjusment of pH value. This test was performed without adjustment of the pH. pH values range from 2.0 to 6.3 at the beginning of the test. At the end of the test the pH was 2.1 to 6.4. The highest tested concentration was 10000 mg/l. The adjusted pH was between 7.5 and 6.3 at the beginning of the test and between 2.1 and 6.4 at the end of the test (24h). The NOEC was 9000 mg/l. The LOEC was 10000 mg/l with 2.5 % inhibition. In the test the stability of the substance was not measured, but the test has been accepted, since in the fish test it was proven, that the substance is stable.