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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes
Details on sampling:
- Concentrations: nominal concentration: 320, 580, 1000, 1800, 3200, 5800, 10 000 mg/l
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION: dispersion treatment; 30 min ultra sound at room temperature; the substance was not completely dissolved in all tested concentration levels.
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Pretreatment: the sludge was washed twice with the autoclaved tap water and diluted 1:3
Test type:
static
Water media type:
brackish water
Limit test:
yes
Total exposure duration:
3 h
Hardness:
-
Test temperature:
22°C
pH:
7.2
Dissolved oxygen:
-
Nominal and measured concentrations:
nominal: 320, 580, 1000, 1800, 3200, 5800, 10 000 mg/l
Details on test conditions:
according guideline
Reference substance (positive control):
yes
Remarks:
copper-(II)-sulphate-pentahydrate
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
1 968 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
483 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
yes
Conclusions:
The substance is non toxic in concentrations <=483 mg/l to activated sludge;
Executive summary:

A Respiration Inhibition test with activated sludge according to OECD Guideline 209 and GLP principles was performed about 3 h with the test substance. The test method was static. Nominal concentrations of the test substance were as follows:

 

320 – 580 – 1000 -1800 – 3200 – 5800 -10000 mg/L

 

The EC50 is 1968 mg/L.

The test substance is not toxic in concentrations <= 483 mg/L to activated sludge of a municipal sewage treatment plant.

Description of key information

A Respiration Inhibition test with activated sludge according to OECD Guideline 209 and GLP principles was performed about 3 h with the test substance. The test method was static. Nominal concentrations of the test substance were as follows:

 

320 – 580 – 1000 -1800 – 3200 – 5800 -10000 mg/L

 

The EC50 is 1968 mg/L.

The test substance is not toxic in concentrations <= 483 mg/L to activated sludge of a municipal sewage treatment plant.

Key value for chemical safety assessment

EC50 for microorganisms:
1 968 mg/L
EC10 or NOEC for microorganisms:
483 mg/L

Additional information