Aluminium tridiethylphosphinate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Name of test material (as cited in study report): Exolit OP 1230 - Molecular formula: C12H30O6P3Al - Molecular weight: 390.3 g/mol - Physical state: Solid, white powder - Analytical purity: 98.7 % - Purity test date: 2008-03-04 - Lot/batch No.: DEH2005977 - Expiration date of the lot/batch: 2012-02-22 - Storage condition of test material: Room temperature protected from moisture and light

Analytical monitoring:

yes

Details on sampling:

- Concentrations: The limit concentration and the control were analytically verified in the fresh media (0 h) and old media (48 h) by determination of the Phosphor (Phosphate) content with a cuvette test via UV-spectrophotometry.
- Sampling method: For the old media, separate replicates without test organisms were prepared and incubated under test conditions until analysis after 48 h.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis, if necessary.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was freshly prepared with dilution water, mixed with an ultraturrax for 10 minutes at 24000 rpm and stirred with approximately 1100 rpm for 48 h at room temperature. Finally the dispersion was adjusted with 1 M NaOH to pH 7 ± 0.2 and filtered with a membrane filter.
- Eluate: Dilution water
- Differential loading: 100 mg/L Limit concentration
- Controls: Dilution water without test item tested under the same conditions as the test groups.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding

ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No

Hardness:

Dilution water at test start: 164 (mg CaCO3/L)

Test temperature:

20.3 °C

pH:

Water Quality Parameters at Test Start (0 h)
(measured in one additional replicate of the limit concentration and the control)
Nominal
Test Item
Concentration
[mg/L] pH-Value

100 7.12
Control 7.91

Water Quality Parameters at the Test End (48 hours)
(measured in all replicates)
Nominal
Test Item
Concentration
[mg/L] pH-Values
Replicates
1 2 3 4
100 7.58 6.96 6.95 6.96
Control 7.50 7.57 7.59 7.58

Dissolved oxygen:

8.6 - 8.7 mg/l

Salinity:

Not measured, freshwater

Details on test conditions:

- Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None

OTHER TEST CONDITIONS
- Adjustment of pH: No


TEST CONCENTRATIONS
- Range finding study: Yes
Immobilization Rates of the Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
Test Item
Concentration
[mg/L] IMMOBILIZATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
100 0 0 0 10 0 5
Control 0 0 0 0 0 0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No

Results with reference substance (positive control):

- Results with reference substance valid? Yes

Reported statistics and error estimates:

The effects of the limit concentration were evaluated directly from the observation data without mathematical calculation.

Immobilization Rates of the Definitive Test after 24 and 48 h of Exposure

                  (n = 20, divided into 4 replicates with 5 daphnids each)

	IMMOBILIZATION [%]
Nominal Test Item Concentration [mg/L]	24 h					48 h
	Replicates					Replicates
	1.	2.	3.	4.	MV	1.	2.	3.	4.	MV
100	0	0	0	0	0	0	0	0	0	0
Control	0	0	0	0	0	0	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

At the limit concentration 100 mg/L (nominal) of the test item no effect was observed.

Executive summary:

In the acute immobilization test toDaphnia magna(STRAUS) the effects of the limitconcentration 100 mg/L of the test item were determined according to OECD 202 (2004). The limit test was conducted under staticconditions over a period of 48 h from 2010-08-02 to 2010-08-06, with the definitiveexposure phase of 48 h from 2010-08-04 to 2010-08-06 at DR. U. NOACK-LABORATORIEN,Käthe-Paulus-Str. 1,D-31157 Sarstedt, Germany .20 test organisms were exposed to the limitconcentration and the control. The limitconcentration and the control were analyticallyverified in the fresh media (0 h) and oldmedia (48 h) by determination of the Phosphor(Phosphate) content witha cuvette test via UV-spectrophotometry. Details ofthe analytical method are given in part 9. The recoveriesof the test item in the limitconcentration were determined tobe 107 % of the nominal valuein the fresh media at test start (0 h) and109 % in the old media at test end (48 h),demonstrating that concentrations of the test item were successfully maintained for theduration of the test. All effect values given are based on the nominal test item concentration.Water quality parameters (pH-value and dissolved oxygen concentration), measured in the fresh (0 h) an old media (48 h), were determined to be within theacceptable limits. The validity criteria of the test guideline were fulfilled.

EC50 nominal > 100 mg/L

NOEC = 100 mg/L

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

test substance stirred with the Ultra-Turrax for 24 h

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.1 - 2.5 Ca2+ - Mg2+/l

Test temperature:

21.2 - 21.4°C

pH:

7.3 - 7.6

Dissolved oxygen:

8.4 - 8.6 mg/l

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Loss of concentration of the test substance over the duration of the test>20%. The test solution revealed clouding. Substance particles were determined on the surface of the water. No immobilization was determined up to the solubility limit.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this test the EC50 of the test item after 48 hours was > 100 mg/L (nominal concentration). No immobility occured up to the solubility limit of the test item in the test water.

Executive summary:

The test substance was tested in Daphnia magna over 48 hours in a static system.

 

The concentration tested were 100 mg/L and a negative control (0 mg/L).

Before insertion of the test organisms the chamber contents were stirred for approximately 24 hours with a magnetic stirrer to ensure that solubility limit of the test substance in the test water is reached.

The aqueous deposits were observed on the water surface.

The substance concentration was determined in the 100 mg/L group. Because of the low water solubility of the test substance in water a concentration of only 66 mg/L was determined at study start. The concentration decreased to approx. 1 mg/L at study and. Dissolved and dispersed undissolved parts of the test substance contributed to the analytical result. The immobility values given in this report are based on nominal concentrations. The results based on the mean of the analytically determined concentrations are given in the brackets.

 

EC50 (48 hours) > 100 mg/L (33.7)

 

No immobility occurred up to the solubility limit of the test substance in the test water.

Description of key information

On Key and one supporting study are avaialbel. In both studies a limit concentration 100 mg/L (nominal) of the test item no effect was observed. No immobility occured up to the solubility limit of the test item in the test water.

In both studies the EC50 48 -hours is > 100 mg/L. The NOEC = 100 mg/L