Aluminium tridiethylphosphinate

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish, juvenile growth test

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 215 (Fish, Juvenile Growth Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Name of test material (as cited in study report): Exolit OP 1230 - Molecular formula: C12H30O6P3Al - Molecular weight: 390.3 g/mol - Physical state: Solid - Analytical purity: 98.7 % - Purity test date: 2008-ß3-04 - Lot/batch No.: DEH2005977 - Expiration date of the lot/batch: 2012-02-22 - Storage condition of test material: Room temperature protected from moisture and light

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/l nominal; the analytical determination included dissolved and dispersed undissolved parts of the test substance;
- Sampling method: test concentration stirred for 24 h;

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances) - Method: A stock solution of 100 mg/L (total volume 20 L) was prepared for each renewal and mixed with an ultraturrax for 10 minutes. This dispersion was stirred for 48 h. Finally the dispersion was adjusted with NaOH (1 M) to pH 7.2 (± 0.1) and filtrated with a RC filter (0.45 μm). This stock solution was used for the preparation of the test media. - Eluate: Dilution water - Differential loading: 1 - 3.16 - 10 - 31.6 - 100 mg/L - Controls: One replicate with 16 fish was tested under the same test conditions as the test item replicates but without test item.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM - Common name: Zebrafish - Source: Aquarium am Aegi, 30175 Hannover, Germany - Weight at study initiation (mean and range, SD): 0.05 - 0.1 g - Method of breeding: Holding was performed at the test facility at 23 +/- 2 °C and diffuse light (0.1-10 μmol photons • m-2 • s-1, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value. Zebrafish with at least 12 days of acclimatisation and mortality < 5 % within the last 7 days before the study started was used in the test. No disease treatment was administered throughout holding and testing. FEEDING DURING TEST - Food type: Brine shrimp nauplii Artemia salina eggs were purchased from Silverstar Artemia Brine Shrimp Ex, Inter Ryba GmbH Germany. Fresh cultures were prepared with salt water (NaCl 40 g/L, 2 g eggs to 1 L salt water, gentle aeration). 24 h old brine shrimp nauplii were harvested and washed with a stainless steel mesh and resuspended in dechlorinated tap water. - Amount: The fish were fed daily ad libitum. The food was given in two feeds per day separated by at least 5 hours (workdays). One feed was applied on weekends

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Hardness:

2.1 - 2.5 mmol Ca2+ + Mg2+/l

Test temperature:

22+/- °C

pH:

7.5 - 7.7

Dissolved oxygen:

6.7 - 9.0 mg/l

Salinity:

Not measured, freshwater

Nominal and measured concentrations:

Analytical evaluation of Phosphor (Phosphate) was carried out via UV-spectrophotometry.
Recovery rate: 83 - 97%

Reference substance (positive control):

no

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth rates and lethal effects

Duration:

28 d

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth rates and lethal effects

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: growth rates and lethal effects

Validity criteria fulfilled:

yes

Conclusions:

In this study the test substance did not cause any effects to Zebrafish (growth rates, lethal and non lethal effects), at nominal test item concentrations up to 100 mg/L. Therefore the NOEC after 28 days of exposure was 100 mg/L (nominal test item concentration).

Executive summary:

The effects on the growth rate of juvenile Zebrafish caused by the testitem Exolit OP 1230(batch no. DEH2005977) were determined according to OECD-Guideline 215 from June 04to July 02, 2008 at DR.U.NOACK-LABORATORIEN, , . A semi-static test with 3 renewals of testsolutions per week was carried out. Five testconcentrations in a geometrical series with adilution factor of 3.16 were tested: 1 3.16 10 31.6 100 mg/L. Duration of the testwas 28 days. 16 fish were exposed to each testdosage level and control. No vehicles wereused for dissolving the test item. Daily observation of the fish wascarried out with respectto lethal and non lethal effects. Body lengthand weight of the fishwere determined on days0 and 28. Water quality parameters temperature, pH value, oxygen saturation weredetermined to be within the acceptable limits (see part 9). Control analysis of phosphorusas phosphate was carried out weekly from freshly prepared and 48 h old solutions viaphotometry (see part 10). The measured phosphorus concentrations were in the range of83 97 % of the nominal values. Nominal test item concentrations were used for theevaluations of the effect levels.

NOEC = 100 mg/L

LOEC > 100 mg/L

EC50 > 100 mg/L

Description of key information

The effects on the growth rate of juvenile Zebrafish caused by the testitem Exolit OP 1230(batch no. DEH2005977) were determined according to OECD-Guideline 215 from June 04to July 02, 2008 at DR.U.NOACK-LABORATORIEN, , . A semi-static test with 3 renewals of testsolutions per week was carried out. Five testconcentrations in a geometrical series with adilution factor of 3.16 were tested: 1 3.1610 31.6 100 mg/L. Duration of the testwas 28 days. 16 fish were exposed to each testdosage level and control. No vehicles wereused for dissolving the test item. Daily observation of the fish wascarried out with respectto lethal and non lethal effects. Body lengthand weight of the fishwere determined on days0 and 28. Water quality parameters temperature, pH value, oxygen saturation weredetermined to be within the acceptable limits (see part 9). Control analysis of phosphorusas phosphate was carried out weekly from freshly prepared and 48 h old solutions viaphotometry (see part 10). The measured phosphorus concentrations were in the range of83 97 % of the nominal values. Nominal test item concentrations were used for theevaluations of the effect levels.

NOEC = 100 mg/L

LOEC > 100 mg/L

EC50 > 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L