Ethyl 2-methylbut-3-enoate

Endpoint summary

Description of key information

SKIN IRRITATION 
No animal data are available. 
In vitro EpiDerm™ Test: Effects indicating an irritant property were observed, but there was no indication of a corrosive property (BASF SE, 2009). 
Classification / labeling: Irritating to the Skin (R 38) 
EYE IRRITATION 
No animal data are available. 
In vitro HET-CAM Test: Effects on chorioallantoic membrane indicating an irritant/corrosive property were observed (BASF SE, 2009). 
Classification / labeling: Risk of serious damage to the eyes (R 41) 
RESPIRATORY IRRITATION 
No data are available.

Additional information

SKIN IRRITATION

No animal data are available.

In an in vitro EpiDerm™ Test (BASF SE, 2009), EpiDerm™ tissue samples were incubated with unchanged, liquid 2-Methylbuten-3-säureethylester (ca. 99% pure) according to the methods of the Corrosion test and the Irritation test. For the corrosion test, two EpiDerm™ tissue samples were incubated with the test item for 3 minutes and 1 hour, respectively. The irritation test was performed with three EpiDerm™ tissue samples, which were incubated with the test item for 1 hour followed by a 42-hours post-incubation period. Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure using a colorimetric test. The reduction of mitochondrial dehydrogenase activity, measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as suitable endpoint. The test was carried out in accordance with OECD TG 431.

2-Methylbuten-3-säureethylester was not able to reduce MTT directly. In the Corrosion test, the mean viability of the test-item treated tissues determined after an exposure period of 3 minutes was 111%, and it was 43% after an exposure period of 1 hour. In the Irritation test, the mean viability of the test-item treated tissues determined after an exposure period of 1hour with about 42 hours post-incubation was 7%. Based on the observed results and applying the evaluation criteria, it was concluded that 2-Methylbuten-3-säureethylester shows a skin irritation potential in the EpiDerm™ skin corrosion/irritation test under the test conditions chosen.

EYE IRRITATION

No animal data are available.

In an in vitro HET-CAM Test (BASF SE, 2009), 2-Methylbuten-3-säureethylester (ca. 99% pure) was applied to the chorioallantoic membrane (CAM) of fertilized and incubated hen eggs, either undiluted or as a 10% solution in olive oil (single topical application of aliquots of 0.3 ml of the respective test liquid). Three eggs per test-substance concentration were observed up to a maximum of 160 seconds and the occurrence of vascular injury or intravascular coagulation in response to the test substance was recorded.

The chorioallantoic membrane of the eggs treated with the 10% test-item solution in olive oil did not show any irritation effects. The undiluted test item caused moderate intravascular coagulation and slight haemorrhagia in all eggs after 36 – 40 seconds. Based on the results of this study and applying the evaluation criteria, it is concluded that 2-Methylbuten-3-säureethylester causes serious eye damage in the HET-CAM Test under the test conditions chosen.

RESPIRATORY IRRITATION

No data are available.

Justification for classification or non-classification

Based on the results of the in vitro EpiDerm™ test, 2-Methylbuten-3-säureethylester has to be classified as follows: Irritating to the Skin (R 38) according to the Directive 67/548/EC. According to the GHS criteria, 2 -Methylbuten-3 -säureethylester has to be classified as follows: Irritating to the skin, Cat. 2.

Based in the results of the in vitro HET-CAM test, 2-Methylbuten-3-säureethylester has to be classified as follows: Risk of serious damage to the Eye (R 41) according to the Directive 67/548/EC. According to the GHS criteria, 2 -Methylbuten-3 -säureethylester has to be classified as follows: Corrosive Cat. 1 (Irreversible effects to the Eye / Serious damage to the eyes).