Ethyl 2-methylbut-3-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: non-GLP Screening Test, however carried out at a GLP lab following OECD testing guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Principles of method if other than guideline:

Screening study similar to OECD 202 guideline.

GLP compliance:

yes

Remarks:

non-GLP-screening test

Test material

Test material information

Constituent 1

Reference substance name:

ethyl (2R)-2-methylbut-3-enoate; ethyl (2S)-2-methylbut-3-enoate

EC Number:

700-224-5

Cas Number:

1647-12-7

Molecular formula:

C7 H12 O2

IUPAC Name:

ethyl (2R)-2-methylbut-3-enoate; ethyl (2S)-2-methylbut-3-enoate

Details on test material:

- Name of test material (as cited in study report): 2-Methylbuten-3-saeureethylester
- Analytical purity: 99.2 w-%
- Lot/batch No.: 34649/199/Fr.3

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was weighed separately for each concentration (differential loading) and stirred in M4 medium for approx. 10 minutes at 20 °C. The stock solutions were clear and colorless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The clone of Daphnia magna was bred at the testing facility.
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guideline 209


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 24 and 48 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility