Butyltris[(2-ethyl-1-oxohexyl)oxy]stannane

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not specified

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Restriction: No analytical confirmation of exposure concentrations.

Cross-reference

Reason / purpose for cross-reference:

other: read-across target

Data source

Reference

Reference Type:

publication

Title:

Toxicity of organotin compounds to activated sludge.

Author:

Stasinakis, A.S., Thomaidis, N.S. and Lekkas, T.D.

Year:

2001

Bibliographic source:

Ecotoxicology and Environmental Safety 49: 275-280.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: other: American Public Health Association, 1989, Standard Methods for Examination of Water and Wastewater, 17th ed.

Short-term toxicity screening test conducted to evaluate the toxicity of organotins to activated sludge and the impact of sludge age on the toxicity of these compounds. Inhibition of respiration rate was chosen as a short-term screening test for the evaluation of toxicity.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N-butyltin trichloride

EC Number:

214-263-6

EC Name:

N-butyltin trichloride

Cas Number:

1118-46-3

Molecular formula:

C4H9Cl3Sn

IUPAC Name:

n-butyltin trichloride

Details on test material:

95% purity

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

See below

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Test conditions

Details on test conditions:

Composition of synthetic wastewater:

Glucose: 787.5 mg/L
(NH4)3PO4: 111.8 mg/L
K2HPO4: 1750 mg/L
KH2PO4: 250 mg/L

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Dose descriptor:

IC50

Effect conc.:

134.5 mg/L

Remarks on result:

other: reported as Ki in study

Reported statistics and error estimates:

Ki = inhibition coefficient (concentration of inhibitor giving half of the maximum inhibition) (mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The inhibition concentration of the test material was 134.5 mg/L.

Executive summary:

The test material is unlikely to inhibit microorganisms in the activated sludge process because the concentrations giving 50 % of the maximum inhibition respiration rate are higher than those often detected in activated sludge treatment plants. The toxicity of the test material is reduced as exposure time increases. The inhibition concentration of the test material was 134.5 mg/L.