Butyltris[(2-ethyl-1-oxohexyl)oxy]stannane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 July 2001 to 3 August 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

DOC analysis only at the beginning of the test was carried out at the sponsor's wish. The test material stability during the test was not checked. Fish with 7 instead of 12 days acclimatisation were used for the test. Other fish were not available.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- The limit test concentration (100 mg/L) and the control were analysed from a sample taken at the start of the test.

Vehicle:

no

Details on test solutions:

An aliquot of dilution water (30 mL) was added to the test material and treated with ultrasound (15 min at room temperature). After application the test medium was mixed with a laboratory blender (20000 rpm, 1 min).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Brachydanio rerio (Zebra fish). Gnathostoma, Pisces, Osteichthyes, Teleostei, Clupeiformes, Cyprinidae
- Length at study initiation: average 2.47 cm
- Weight at study initiation: average 0.22 g

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: Holding was performed at the test facility at 23 ± 2 °C and diffuse light (0.1-10 μmol/m^2s, natural photoperiod) in tap water. The water was changed at least once per week. The dissolved oxygen concentration was more than 80% of the air saturation value.
- Type and amount of food during acclimation: Food was provided 3 times per week. The amount of food was 4% of the fish body weight per feeding day. The test fish were not fed until 24 h before the test started. The diet was Trouvit 40 / 2.
- Health during acclimation (any mortality observed): mortality was < 5 %. No disease treatments were administered throughout holding.

FEEDING DURING TEST: No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

62 mg CaCO3/L

Test temperature:

22.2 - 22.9 °C

pH:

7.04 - 7.70

Dissolved oxygen:

82 - 91 % saturation

Nominal and measured concentrations:

Nominal: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass-aquaria loosely covered by glass tops were used
- Fill volume: 10 L
- Aeration: Gentle aeration was provided
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: Fish density in the tanks was less than 1 g fish per litre test solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine. The water is analysed once a year acc. to German tap water regulation.
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH-value, temperature and oxygen saturation were measured in all vessels at the beginning of the test and every 24 h. Total hardness of the water was determined at the beginning of the test out of the test control. During the test the room temperature was recorded continuously with a thermohygrograph.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: A natural photoperiod was provided during the test
- Light intensity: 0.1 - 10 μmol/m^2.s

EFFECT PARAMETERS MEASURED:
- Observations were made after 24, 48, 72 and 96 h
- Fish would have been considered dead if there was no visible movement (e.g. gill covers movement) and if touching of the caudal peduncle produced no reaction. Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.)
- At test start body weight and total length of a representative number of 10 test fish were determined. These fish were not used for testing
- The concentration leading to 0 and 100% mortality (LC0 and LC100) as well as the no effect level (NOEC) after 96 h were determined directly from the test results. LC50-values have not to be calculated in a limit test

TEST CONCENTRATIONS
- Based on the results of a preliminary range finding test the definitive study was performed as a limit test with 100 mg/Las single test concentration

VALIDITY CRITERIA
- O2 saturation ≥ 60%
- Mortality in the control ≤ 1/7 fish

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

PRELIMINARY TEST
- No mortality was observed in the preliminary test at 100 mg/L.

MAIN TEST
- 14% mortality in the test concentration of 100 mg/L (nominal concentration) are regarded to be not significant. No non lethal effects were observed throughout the test. Therefore the NOEC was laid down as 100 mg/L (nominal concentration).

VALIDITY CRITERIA
The validity criteria were fulfilled:
- O2 - saturation was ≥ 60%
- Mortality in the control was 0%

Sublethal observations / clinical signs:

Table 1: Observations in the Test Vessels

Concentration (mg/L)	Effect	Test Duration (h)
		24	48	72	96
100	(E)	-	-	-	1/7
	(1)	7/7	7/7	7/7	6/7
Control	(1)	7/7	7/7	7/7	7/7

(1) = Normal behaviour

(E) = Exitus lethalis

Table 2: Cumulative Mortality in [%] in the Test Vessels

Concentration (mg/L)	Test Duration (h)
	24	48	72	96
100	0	0	0	14
Control	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study, the 96-hour LC50 was determined to be in excess of 100 mg/L.

Executive summary:

The acute toxicity of the test material to fish was investigated in accordance with the standardised guideline OECD 203, under GLP conditions.

A static limit test was conducted using Brachydanio rerio (Zebra fish) with the nominal concentration 100 mg/L selected on the basis of a preliminary range finding test, the duration of the test was 96 hours. During the study 7 test organisms were exposed to the test concentration and the control.

The test material showed sedimentation and turbidity throughout the test. All effect values are based on the nominal concentration of the test material. DOC-analysis was carried out at test start from the test material and the control vessel.

Water quality parameters of temperature, pH-value, oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. The validity criteria of the test were fulfilled.

14% mortality in the test concentration of 100 mg/L (nominal concentration) were regarded to be not significant. No non-lethal effects were observed throughout the test.

Therefore, under the conditions of this study, the test material was not found to be toxic to Zebra fish after 96 h at the nominal concentration 100 mg/L.

Description of key information

Under the conditions of this study, the 96-hour LC50 was determined to be in excess of 100 mg/L.

Additional information

The acute toxicity of the test material to fish was investigated in accordance with the standardised guideline OECD 203, under GLP conditions.

A static limit test was conducted using Brachydanio rerio (Zebra fish) with the nominal concentration 100 mg/L selected on the basis of a preliminary range finding test, the duration of the test was 96 hours. During the study 7 test organisms were exposed to the test concentration and the control.

The test material showed sedimentation and turbidity throughout the test. All effect values are based on the nominal concentration of the test material. DOC-analysis was carried out at test start from the test material and the control vessel.

Water quality parameters of temperature, pH-value, oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. The validity criteria of the test were fulfilled.

14% mortality in the test concentration of 100 mg/L (nominal concentration) were regarded to be not significant. No non-lethal effects were observed throughout the test.

Therefore, under the conditions of this study, the test material was not found to be toxic to Zebra fish after 96 h at the nominal concentration 100 mg/L.